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SOLVED: At 260 nm, NAD and NADH both absorb and have an extinction coefficient of 1.8 x 10^4 M^-1 cm^-1. NAD does not absorb at 340 nm. The extinction coefficient of NADH at 340 nm is 6.2 x 10^3 M^-1 cm^-1. The absorbances were measured in a cell with a path length of 1 cm. Using the Beer-Lambert Law, calculate the concentrations of NAD and NADH in the solution in units of μmol per liter (μM). Show working out and answer in M and μM. What does [NADH] [NAD ] and [NADH], and [NAD ] equal?
SOLVED: At 260 nm, NAD and NADH both absorb and have an extinctio…
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