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SOLVED: 12-11 The structures of Ran-GDP and Ran-GTP (actually Ran-GppNp, a stable GTP analog) are strikingly different, as shown in Figure Q 12-2 . Not surprisingly, Ran-GDP binds to a different set of proteins than does Ran-GTP. To look at the uptake of Ran itself into the nuclei of permeabilized cells, you attach a red fluorescent tag to a cysteine side chain in Ran to make it visible. This modified Ran supports normal nuclear uptake. Fluorescent Ran-GDP is taken up by nuclei only if cytoplasm
SOLVED: 12-11 The structures of Ran-GDP and Ran-GTP (actually R…
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