Sulfhydryl Group | Structure, Formula & Examples - Video | Study.com
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What is a sulfhydryl group, and how does it interact biochemically with heavy metals? How does the interaction affect processes in the body? | Numerade
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SOLVED:VISUAL SKILLS Which functional group is present in this molecule? (A) sulfhydryl (B) carboxyl (C) methyl (D) phosphate
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Sulfhydryl groups have the ability to react with the alkylating reagent N-ethylmaleimide (NEM) in chemical modification experiments. When NEM is added to a purified solution of creatine kinase, Cys 278 is alkylated, but no other Cys residues in the protein are modified. What can you infer about the Cys 278 residue based on this observation?
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An enzyme that catalyzes disulfide-sulfhydryl exchange reactions, called protein disulfide isomerase (PDI), has been isolated. PDI rapidly converts inactive scrambled ribonuclease into enzymatically active ribonuclease. In contrast, insulin is rapidly inactivated by PDI. What does this important observation imply about the relation between the amino acid sequence of insulin and its three-dimensional structure? | Numerade
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