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SOLVED: RNase P is an essential enzyme that processes the 5' end leader sequence of pre-tRNA to generate mature tRNA. The bacterial RNase P contains an RNA subunit and one protein subunit, where the RNA subunit contains the catalytic activity. The protein subunit lacks any catalytic activity but relaxes the ionic requirements for holoenzyme reaction and is indispensable for pre-tRNA cleavage in vivo. In the current study, we reconstituted the M. tuberculosis RNase holoenzyme in vitro. We prepare
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