Overall, our observations highlight a bidirectional function of the Rac1:PKA complex. GTP-Rac1 enriches and compartmentalizes inactive PKA complexes through binary interaction with RII subunits.

GPCR-triggered cAMP-elevation promotes PKA dissociation and PKAc phosphorylation of GTP-Rac1-bound and GTP-Rac1-activated PAK which contributes to elevations of downstream signaling. Previously, we demonstrated that cellular PPI between PKA subunits and diverse GTPases is not static.

Oct 14, 2014 · The PKA-Rac1 axis functions to activate ERK MAP kinase and thereby regulates cell proliferation. It should be studied further whether the PKA-Rac1 axis functions also in neuronal cells.

Nov 8, 2021 · We found that Rac1/Cdc42 phosphorylation was inhibited by CDH12 knockdown and could be rescued by PKA agonists. Taken together, our study revealed that CDH12 can influence neurite outgrowth in a PKA/Rac1/Cdc42 dependent manner.

Oct 28, 2024 · These pro-angiogenesis activities were abrogated by highly specific cell-permeable peptide inhibitor (PKI) of PKA. Activation of cAMP/PKA-signalling resulted in an increased VEGFR2 and Akt phosphorylation, increased Rac1 activity, and a …

We demonstrated that GTP-activated Rac acts as a dual kinase-tuning scaffold for p21-activated kinase (PAK) and PKA activities. We showed that receptor-triggered PKA trans-phosphorylation of GTP-Rac-organized PAK contributes to elevations of nuclear Erk1/2 signaling and proliferation.

We report that Rac1, a member of the Rho GTPase family (25), contains AKAP properties and, thus, show direct interactions with PKA R subunit type II (RII) in vitro and in β β vivo. Our cell-based studies demonstrate that complex formation of active GTP-Rac1 and PKA increase inactive PKA complexes but does not directly affect Rac1 GTPase activity.

May 21, 2013 · The cAMP-dependent protein kinase A (PKA) is the prime example for compartmentalized signal transmission downstream of distinct GPCRs. A-kinase anchoring proteins tether PKA to specific intracellular sites to ensure precision and directionality of PKA phosphorylation events.

Recently, we have identified dynamic interaction and reciprocal regulation of cAMP-dependent protein kinase A (PKA) and Rho-GTPase Rac signaling. We demonstrated that GTP-activated Rac acts as a dual kinase-tuning scaffold for p21-activated kinase (PAK) and PKA activities.

Nov 8, 2021 · The transcriptome profile of neurons with or without siCDH12 treatment revealed a set of pathways positively correlated with the effect of CDH12 on neurite outgrowth. We further revealed that CDH12 affected Rac1/Cdc42 phosphorylation in a PKA-dependent manner after testing using H-89 and 8-Bromo-cAMP sodium salt.