Dec 2, 2019 · Live-cell imaging of the U-2 OS YAP–HaloTag cell line. The YAP–HaloTag U-2 OS cells were pre-plated on eight-well LabTek chambered coverglass dishes for at least 16 h in complete medium.

Plasmid pQCXIP-mCherry-Halo-YAP1 from Dr. Yutaka Hata's lab contains the insert YAP1 and is published in Mol Cancer Res. 2018 Feb;16 (2):197-211. doi: 10.1158/1541-7786.MCR-17-0382. Epub 2017 Oct 23. This plasmid is available through Addgene.

To test how YAP condensation can be regulated by other physiologically relevant signals, we utilized a U-2 OS cell line where the endogenous YAP protein is labeled with a HaloTag (U-2 OS YAP-HaloTag, Figure 1A), in which YAP-HaloTag forms liquid-like nuclear condensates at endogenous YAP expression levels, 10 colocalizing with YAP target gene ...

Aug 10, 2024 · Based on this work, we analyze YAP in spontaneously differentiating mESCs at 1.5–2 d post differentiation start. To visualize chromatin-bound YAP molecules, we leverage single-molecule imaging of sparsely labeled (Halo-tag JFX549) cells.

Jun 21, 2024 · Here, we report that endogenous YAP forms sub-micron scale con-densates in response to Hippo pathway regulation and actin cytoskeletal tension. YAP condensates are stabilized by the transcription factor TEAD1, and recruit BRD4, a coactivator that is enriched at active en-hancers.

Aug 19, 2021 · Expression of the epithelial to mesenchymal transcription factor Snail displaced Scrib-HaloTag from cell junctions, mirroring the mislocalization observed in cancers. Interestingly, Snail expression promotes Yes-associated protein-1 (YAP1) nuclear localization independent of hippo pathway-regulated YAP-S127 phosphorylation.

Yes-associated protein (YAP) is a transcriptional co-activator that regulates cell proliferation and survival by binding to a select set of enhancers for target gene activation. How YAP...

May 1, 2024 · YAP protein is labeled with a HaloTag (U-2 OS YAP-HaloTag, Figure 1 A), in which YAP-HaloTag forms liquid-like nuclear condensates at endogenous YAP expression levels, 10

Jun 21, 2024 · To test how YAP condensation can be regulated by other physiologically relevant signals, we utilized a U-2 OS cell line where the endogenous YAP protein is labeled with a HaloTag (U-2 OS YAP-HaloTag, Figure 1A), in which YAP-HaloTag forms liquid-like nuclear condensates at endogenous YAP expression levels,10 colocalizing with YAP target gene MYC...

HaloTag is a self-labeling protein tag. It is a 297 residue protein (33 kDa) derived from a bacterial enzyme, designed to covalently bind to a synthetic ligand. The bacterial enzyme can be fused to various proteins of interest. [1] .