Nov 10, 2017 · 4-AP is used to block K + channel-mediated shunting of the light-evoked response. This increases the membrane space constant and raises the odds that the light pulse translates to...

tetrodotoxin（TTX）作为电压门控型钠离子通道阻断剂，施加光刺激，此时没有动作电位。 若附加4-AP来阻断钾离子通道，以减小正离子外流的影响，就观测到了动作电位。 就可以证明两脑区之间是单突触连接

Using a laser scanning system (and adding TTX and 4AP in the bath solution) allows high-resolution mapping of the dendritic location of presynaptic inputs, as discussed above (Petreanu et al. 2009).

Nov 10, 2020 · As such, we can distinguish monosynaptic connections from multisynaptic ones by adding TTX to the bath. This is often accompanied by 4AP to block delayed rectifier potassium currents.

Jun 1, 2020 · Optogenetically evoked PSC (in TTX/4AP, blue trace) were nearly completely eliminated by 40 μM PTX (+PTX, red trace). Scale bars, 100 pA and 100 ms.

TTX blocks action potentials and abolishes both mono- and polysynaptic (opto-evoked) neurotransmission. In the case of a monosynaptic response, the amplitude of the opto-evoked synaptic transmission will be (at least partially) rescued by the addition of 4-AP, which prolongs the depolarization of local presynaptic terminals, boosting vesicle ...

Jun 15, 2017 · EPSCs were abolished after bath application of TTX (1 mM) but were then reintroduced following bath application of 4-AP (100 mM; in the presence of TTX), a voltage-dependent potassium (K+)...

Nov 5, 2024 · We first demonstrated that avTRN-evoked IPSCs in aPVT neurons were abolished by the bath application of the sodium channel blocker tetrodotoxin (TTX) and restored by the additional application of the potassium blocker 4-aminopyridine (TTX + 4AP), confirming that they were caused by action potential firing in presynaptic terminals from avTRN ...

Mar 4, 2015 · IPSCs could be rescued in the continued presence of TTX by enhancing ChR2-mediated depolarization of terminals with application of the voltage-gated K + channel blocker 4-aminopyridine...

Mar 16, 2021 · Pie charts report that all recorded neurons exhibited a monosynaptic GABAergic transmission, blocked by TTX and reappearing with 4AP. (I) Monosynaptic glutamatergic currents are also acquired in all recorded Pf thalamic cells.