Jul 29, 2005 · Sinusoids of the spleen and liver were diffusely positive for CD31. They were negative for CD34 in the spleen and only expressed CD34 in the periportal area in the liver. Fli-1 was expressed in all types of EC but also in lymphocytes.

CD31 is a marker of vascular endothelium [57], and the CD31 + cells are depicted as brown (brown arrow) by immunohistochemical staining, showing new blood vessels. A little bone formation with ...

Jan 7, 2022 · We found analogous fibroblastic stromal cell diversity in the human spleen. In addition, we identify the transcription factor SpiB as a critical regulator required to support white pulp FRC differentiation, homeostatic chemokine expression, and antiviral T cell responses.

Mar 27, 2019 · In fact, their immunophenotype is so distinctive, that if present, it can identify splenic tissue in unusual sites (ie, ectopic/heterotopic spleen). Splenic littoral cells express endothelial markers such as CD31, Wilms tumor protein 1 (WT1), factor VIII, and ERG, as well as CD68 and variably, CD21. 5,6 Their most distinctive marker, though, is ...

Jul 17, 2020 · Triggering of MHC molecules by migrating T-cells is a minimal signal capable of inducing endothelial contraction and transient microvascular leakage. Using this model, we show that EB recovery...

Sep 17, 2021 · Timing: ∼6 h 15 min. For preparation of spleen digestion buffer, 45 min. For digestion of spleen tissue, 2 h. For immune cell depletion using CD45 murine MicroBeads, 45 min. For endothelial cell enrichment using CD31 murine MicroBeads, 45 min. For FACS, 1–2 h.

Sinusoids of the spleen and liver were diffusely positive for CD31. They were negative for CD34 in the spleen and only expressed CD34 in the periportal area in the liver.

Sinusoids of the spleen and liver were diffusely positive for CD31. They were negative for CD34 in the spleen and only expressed CD34 in the periportal area in the liver. Fli-1 was expressed in all types of EC but also in lymphocytes.

Aug 29, 2013 · We found p110δ is expressed in gp38 − CD31 + and gp38 + CD31 + spleen stromal cell subpopulations, where it regulates LTβR expression as well as CCL19 and CCL21 production; this suggests a role for p110δ in the control of T cell migration to appropriate spleen areas through the regulation of homeostatic chemokine production by stromal cells.

Aug 29, 2013 · The role of p110δ PI3K in lymphoid cells has been studied extensively, showing its importance in immune cell differentiation, activation and development. Altered T cell localization in p110δ-deficient mouse spleen suggested a role for p110δ in non-hematopoietic stromal cells, which maintain hematopo …