Short hairpin RNA - Wikipedia
A short hairpin RNA or small hairpin RNA (shRNA/Hairpin Vector) is an artificial RNA molecule with a tight hairpin turn that can be used to silence target gene expression via RNA …
Short Hairpin RNA (shRNA): Design, Delivery, and Assessment of …
We provide suggestions for designing shRNA targets and controls, a protocol for sequencing through the secondary structure of the shRNA hairpin structure, and protocols for packaging …
The RNAi Consortium shRNA Library - Broad Institute
The RNAi Consortium, or TRC, is a public-private effort based at the Broad whose mission is to create a shRNA library as well to validate tools and methods that will enable the scientific …
shRNA - Applications - What is shRNA, how it works and its …
Short hairpin RNA (shRNA) sequences are usually encoded in a DNA vector that can be introduced into cells via plasmid transfection or viral transduction. shRNA molecules can be …
Guidelines for the optimal design of miRNA-based shRNAs
Here, based on current understanding of the miRNA maturation pathway, we discuss the principles of different shRNA design (pre-miRNA-like, pri-miRNA-like and Ago-shRNA) with an …
A streamlined method for the design and cloning of shRNAs into …
Short hairpin RNA (shRNA) is an established and effective tool for stable knock down of gene expression. Lentiviral vectors can be used to deliver shRNAs, thereby providing the ability to …
siRNA vs. shRNA: similarities and differences - PubMed
Jul 25, 2009 · Methods of mediating the RNAi effect involve small interfering RNA (siRNA), short hairpin RNA (shRNA) and bi-functional shRNA. The simplicity of siRNA manufacturing and …
Short Hairpin RNA - an overview | ScienceDirect Topics
Short hairpin RNA (shRNA) is an alternative way to prepare siRNA sequences for delivery to cells that can be expressed in situ from plasmid DNA (pDNA) or from virus-derived constructs. The …
siRNAs and shRNAs: Tools for Protein Knockdown by Gene …
Oct 29, 2022 · Two key approaches to RNAi that have gained substantial interest for use in gene silencing are the double-stranded small interfering RNAs (siRNAs) and the vector-based short …
手把手教学｜shRNA设计步骤及注意事项 - 知乎 - 知乎专栏
短发夹RNA（shRNA）是一种具有环状结构的分子，其设计合成的序列能够在细胞内转录和产生与目标mRNA特定区域互补的 siRNA。 这些siRNA与目标mRNA结合，通过 RNA干扰 （RNAi） …