To enable ultrastructural characterization of islet primary cilia, we used surface topography imaging by scanning electron microscopy (SEM) to directly visualize the ciliary axoneme in human pancreatic islets.

Apr 19, 2022 · a Scanning electron microscopy (SEM) revealed that microridges form at stage 26, and continued to increase in complexity towards stage 40, when cilia start to retract (scale bars, 1 µm).

Nov 6, 2023 · Water freeze-drying of biological samples has long been considered unfeasible as a sample preparation method for scanning electron microscopy (SEM) owing to the formation of damaging ice crystals.

Scanning electron microscopy (SEM) is a high-resolution technique uniquely suited for examining the native 3D conformation of surface structures, an approach that we recently used to characterize the primary cilia axoneme of human islets (Polino et al., 2023).

Aug 1, 2022 · In this study, a method was developed to freeze-dry the heterotrichous ciliate Spirostomum ambiguum from water without chemical fixation. The method allowed SEM observation of cells in both elongated and contracted states with …

Feb 15, 2023 · Scanning electron microscopy (SEM) is a useful technique for studying the surface morphology of membrane projections like primary cilia, but conventional sample preparation does not reveal the sub-membrane axonemal structure which holds key implications for cilia function.

To address this question, we examined its ultrastructure by focused ion beam-scanning electron microscopy (FIB-SEM), an EM technique capable of imaging large volumes, several cells wide, with isotropic resolution (Figure 3 A) (Kizilyaprak et al., 2019).

Nov 7, 2023 · Water freeze-drying of biological samples has long been considered unfeasible as a sample preparation method for scanning electron microscopy (SEM) owing to the formation of damaging ice...

Water freeze-drying of biological samples has long been considered unfeasible as a sample preparation method for scanning electron microscopy (SEM) owing to the formation of damaging ice crystals.

We aimed to freeze-dry the ciliate Spirostomum ambiguum, obtained from water, without fixation and observe it using scanning electron microscopy (SEM). Living cells were placed on a specimen stub and frozen upon contact with a Cu block kept at either -80 °C or -100 °C.