The SRA test is a functional assay that measures heparin-dependent platelet activation. Patient serum is incubated with donor platelets containing radiolabeled serotonin and different concentrations of heparin.

Mar 5, 2025 · The SRA employs washed donor platelets and detects their activation by measuring the release of endogenous serotonin induced by addition of patient serum. The serotonin levels are measured by a highly sensitive liquid chromatography/tandem mass spectroscopy method.

Dec 10, 2011 · Three types of assays are highly sensitive for the diagnosis of HIT: the washed platelet activation assays (SRA and HIPA), the IgG-specific PF4-dependent EIAs (EIA-IgG), and the polyspecific EIAs that detects anti-PF4/heparin antibodies of the 3 major immunoglobulin classes (EIA-IgG/A/M).

Serotonin Release Assay (SRA), Unfractionated Heparin - Any patient with an unexplained fall in the platelet count of >40% during or after cessation of heparin therapy should be suspected of hit-heparin induced thrombocytopenia.

Utilizing testing capabilities of two separate laboratory facilities, Mayo Clinic Laboratories’ new serotonin release assay (SRA) is a gold-standard test supported by decades of clinical hematopathologic experience and advanced mass spectrometry testing.

Heparin-induced thrombocytopenia (HIT) is a prothrombotic drug reaction caused by platelet-activating anti-platelet factor 4 (PF4)/heparin antibodies. Pathogenic HIT antibodies can be detected by the serotonin-release assay (SRA), a platelet activation test.

In a negative serotonin release assay (SRA), patient serum induced serotonin release of below 20% from normal donor platelets in presence of low concentration of unfractionated heparin (UFH) (0.1U/mL) regardless of the serotonin release with high concentration of UFH (100 U/mL).

Few laboratory tests are as clinically useful as The platelet serotonin-release assay (SRA): a positive SRA in the appropriate clinical context is virtually diagnostic of heparin-induced thrombocytopenia (HIT), a life- and limb-threatening prothrombotic disorder caused by anti-platelet factor 4 (PF4 …

SRA is often useful for confirmation of weak or “inconclusive” results obtained with the highly sensitive PF4/ELISA. The SRA can also be used to evaluate samples for antibodies that cross-react with low molecular weight heparins such as Lovenox.

Gold standard test for diagnosis of heparin-induced thrombocytopenia (HIT). Indicates whether a test has been approved by the New York State Department of Health. This test is New York state approved. Patient Preparation Instructions patient must follow before/during specimen collection. Collect Specimen type to collect.