SH-SY5Y is a human derived cell line used in scientific research. The original cell line, called SK-N-SH, from which it was subcloned was isolated from a bone marrow biopsy taken from a four-year-old female with neuroblastoma. SH-SY5Y cells are often used as in …

The SH-SY5Y cell line is a thrice cloned subline of the neuroblastoma cell line SK-N-SH (ATCC HTB-11), which was established in 1970 from a metastatic bone tumor from a 4-year-old cancer patient. Applications include use as a transfection host or in immunology, neuroscience, and toxicology research.

Transformed neuronal-like cell lines can be used in vitro to overcome this limitation. However, several caveats exist when utilizing cells derived from malignant tumors. In this context, the popular SH-SY5Y neuroblastoma cell line and its use in in vitro systems is described.

SH-SY5Y is a thrice-subcloned cell line derived from the SK-N-SH neuroblastoma cell line. It serves as a model for neurodegenerative disorders since the cells can be converted to various types of functional neurons by the addition of specific compounds.

Sep 8, 2021 · The SH-SY5Y cell line is a comparatively homogeneous neuroblast-like cell line, which exhibits neuronal marker enzyme activity (tyrosine and dopamine-β-hydroxylases), specific uptake of norepinephrine (NA), and expresses one or more neurofilament proteins. SH SY5Y cells also express opioid, muscarinic, and nerve growth factor receptors.

In this study, we present an optimized neuronal differentiation protocol for SH-SY5Y that requires only two work steps and 6 days. After differentiation, the cells present increased levels of ATP and plasma membrane activity but reduced expression of energetic stress response genes.

The neuroblastoma cell line SH-SY5Y has been a well-established and very popular in vitro model in neuroscience for decades, especially focusing on neurodevelopmental disorders, such as Parkinson’s disease.

人神经母细胞瘤细胞（SH-SY5Y） 是于1970年建自骨瘤转移灶的神经母细胞瘤 SK-N-SH细胞系经3次克隆后的亚系 (SK-N-SH→SH-SY→SH-SY5→SH-SY5Y)。 该细胞的密度可高达106 cells/cm2，具有中等水平的 多巴胺β羟化酶 的活性。 基本形态上皮样，多数贴壁，少数悬浮； 营养体系：推荐 DMEM：F12 (abs9561)+10% FBS (abs972)+1% P/S （abs9244）。 表1 SH-SY5Y细胞基本信息. 首先，我们讨论的是SY5Y五天传一代算不算正常？

The SH-SY5Y human neuroblastoma cell line (ATCC® CRL-2266™) is one of the most used cell lines in neurosciences, either undifferentiated or differentiated into neuron-like cells.

SH-SY5Y is a human-derived cell line that originated from subcloning of the SK-N-SH neuroblastoma cell line in 1970. The parent cell line, SK-N-SH, was developed from the bone marrow biopsy of a four-year-old female neuroblastoma patient.