Nov 18, 2024 · Here, by using single-nucleus RNA sequencing, we show that both human and mouse adipose tissues retain cellular transcriptional changes after appreciable weight loss.

To optimize the ATAC-seq method to frozen adipose tissue and gain high data reproducibility in various tissues, we modified the Omni-ATAC protocol to include an effective bead-based …

Nov 2, 2023 · Avilar ASGPR Ligands Retain Key pH/Ca2+-Dependent Binding •Both pH and Ca 2+ data consistent with ligand release mechanism in early endosome, enabling ASGPR …

The ENCODE ATAC-seq pipeline is used for quality control and statistical signal processing of short-read sequencing data, producing alignments and measures of enrichment. Pipeline …

We outline a modified version of the “Nuclei Isolation for Single Cell ATAC Sequencing” (CG000169 Rev D) user guide, where we fix and permeabilize cells to retain mitochondria and …

Jan 1, 2025 · ATAC-seq (Assay for Transposase-Accessible Chromatin using sequencing) is a method to study genome accessibility. It enables researchers to identify regions of the genome …

ATAC seq aims to capture the open chromatin parts of the genome and throws light on the effect of chromatin packaging on gene expression and gene regulation. ATAC sequencing uses a …

Nov 16, 2020 · Chromatin accessibility mapping is a powerful approach to identify potential regulatory elements. A popular example is ATAC-seq, whereby Tn5 transposase inserts …

ATAC-seq is a versatile technique with a wide range of applications in epigenetic research. It provides valuable insights into nucleosome positioning and chromatin structure, which are …

Jan 10, 2024 · Chromatin accessibility assays may be used to detect epigenetic changes in cardiac lineages during heart development, chromatin landscape changes during aging, and …