RNase 4 is a single-stranded endoribonuclease that cleaves RNA at uridine-purine (U/R) dinucleotide sites. Endoribonuclease activity tolerates common RNA chemical modifications. Enhance analysis of mRNA 5′ cap identity and distribution.

RNase 4 features a unique structure compared to its homologous enzymes in the superfamily. It contains 119 amino acid residues making it the shortest known human RNase and contains no N-glycosylation sites.

Jul 10, 2024 · Ribonuclease 4 (Rnase4) is a potential antimicrobial protein with no known function in the intestines. Here we find that RNASE4 is expressed in intestinal epithelial cells (IEC) including...

Mar 30, 2025 · RNASE4 (Ribonuclease A Family Member 4) is a Protein Coding gene. Diseases associated with RNASE4 include Amyotrophic Lateral Sclerosis 9 and Frontotemporal Dementia And/Or Amyotrophic Lateral Sclerosis 7. Gene Ontology (GO) annotations related to this gene include nucleic acid binding and RNA nuclease activity.

Ribonuclease 4 functions as an intestinal antimicrobial protein to maintain gut microbiota and metabolite homeostasis. Ribonuclease 4 is associated with aggressiveness and progression of prostate cancer.

RNase 4 Digestion and 3´ End Repair Mix (NEB #M1288) is a coformulation of RNase 4 (cut sites: U/A and U/G) and T4 Polynucleotide Kinase that simplifies RNase 4 digestion products into a pool of U-ending RNA oligonucleotides with a homogeneous 3´-hydroxy termini.

Nov 27, 2024 · Data show that the transcription of angiogenin (ANG) and ribonuclease 4 (RNASE4) promoter is influenced by RNA polymerase III (Pol III) elements and could be differentially regulated by an intragenic CCCTC binding factor (CTCF)-dependent chromatin loop.

Jun 25, 2022 · RNASE4 protein in prostate cancer tissues is enhanced and can differentiate prostate cancer and BPH. RNASE4 stimulates prostate cancer cell proliferation, induces tumor angiogenesis, and...

Oct 14, 2022 · We deployed hRNase 4 to characterize mRNAs fully substituted with 1-methylpseudouridine (m1Ψ) or 5-methoxyuridine (mo5U), as well as mRNAs selectively depleted of uridine-two key strategies to reduce synthetic mRNA immunogenicity.

The structural and enzymatic properties of RNase 4 are reviewed. This RNase shows a much higher interspecies similarity (approximately 90%) than the other members of the RNase A superfamily. The enzyme is ubiquitous, with the highest amounts present in liver and lung.