RNA plasmids are non-infectious extrachromosomal linear RNA replicons, both encapsidated and unencapsidated, which have been found in fungi and various plants, from algae to land plants. In many cases, however, it may be difficult or impossible to clearly distinguish RNA plasmids from RNA viruses and other infectious RNAs.

Sep 12, 2023 · A crucial element in plasmid replication is a small RNA molecule termed RNA I that has a pivotal role in regulating the replication process. In this Journal Club article, I highlight an...

These plasmids provide the tools for the simple, rapid creation of new RNA-coding plasmids to produce milligram quantities of homogeneous in vitro transcripts for all applications.

Jan 1, 1989 · In fungi and higher plants, there exists a wide array of resident, nonhorizontally transmissible RNA molecules that replicate in an RNA-dependent fashion. The replication of these molecules is autonomous of both nuclear chromosomes and the genomes of organelles, and is described as plasmids.

This review provides a comparison of the theoretical issues and experimental findings for plasmid DNA and mRNA vaccine technologies. While both have been under development since the 1990s, in recent years, significant excitement has turned to mRNA ...

The method provided baseline separation of isoforms and yielded SC-rich fractions (>90%) virtually free from RNA with levels of genomic DNA (1.3 ± 0.3% µg gDNA/µg SC plasmid DNA) and protein (4.97 ± 1.34 µg/mL) impurities within target specifications.

RNA plasmids are found as both single-stranded and double-stranded forms and replicate in a manner similar to certain RNA viruses. The RNA plasmid encodes RNA-dependent RNA polymerase that directs its own synthesis. Unlike RNA viruses, RNA plasmids do not contain genes for coat proteins.

To understand how a plasmid works, scientists use a plasmid map. This map shows key parts of the plasmid, like where it starts copying itself and any genes it carries. Below are key steps for effectively interpreting a plasmid map: 1. Identify the Origin of Replication (ORI) First, locate the ORI to understand the plasmid's copy number and host ...

Digest plasmid with BsmBI and ligate RNA motif of interest. The RNA motif of interest would be in the 3UTR and flanked by BoxB motifs. Oligo cloning can be performed similarly to sgRNA cloning.

In summary, rNA I has a pivotal role in the replication of ColE1-type plasmids by regulating the activity of rNA II. Its mecha-nism of action and the factors that influ-ence its...