Apr 1, 2002 · Small RNA molecules have been found to be specifically associated with posttranscriptional gene silencing (PTGS) in both plants and animals. Here, we find that small sense and antisense RNAs are also involved in PTGS in Neurospora crassa.

Apr 28, 2010 · Immunoprecipitation of QDE-2 from DNA damaged Neurospora cultures revealed a novel type of small RNAs known as QDE-2-interacting small RNAs that are mostly derived from the ribosomal DNA (rDNA) locus (27). qiRNA production is dependent on QDE-1, QDE-3, and the DCLs but not on QDE-2.

May 14, 2009 · Here we show that DNA damage induces the expression of the Argonaute protein QDE-2 and a new class of small RNAs in the filamentous fungus Neurospora crassa.

Here, by analyzing small RNAs associated with the Neurospora Argonaute protein QDE-2, we show that diverse pathways gen-erate miRNA-like small RNAs (milRNAs) and Dicer-independent small interfering RNAs (disiRNAs) in this filamentous fungus.

Apr 1, 2002 · Supporting this idea, a purified QDE2 protein complex was found to contain small RNA molecules, suggesting that QDE2 could be part of a small RNA-directed ribonuclease complex involved in sequence-specific mRNA degradation.

May 11, 2012 · Here, we reconstitute this Argonaute-dependent sRNA biogenesis pathway in vitro and discover that the RNA exosome is also required for milR-1 production. Our results demonstrate that QDE-2 mediates milR-1 maturation by recruiting exosome and QIP and by determining the size of milR-1.

Analysis of small RNA accumulation in qde mutants. (A) RNA blot hybridization of low-molecular-weight RNAs prepared from the 107 strain (qde-1−), the 820 strain (qde-2−), the 627 strain (qde-3−), the untransformed wild-type strain (unsilenced), and an al-1 silenced strain (6xw). Sense and antisense probes are the same as in Figure 1.

Sep 23, 2022 · Here, we report five complex structures of N-terminal truncated QDE-1 (QDE-1ΔN), representing four different reaction states: DNA/RNA-templated elongation, the de novo initiation of RNA synthesis, the first step of nucleotide condensation during de novo initiation and initial NTP loading.

Aug 30, 2022 · Here, we report five complex structures of N-terminal truncated QDE-1 (QDE-1ΔN), representing four different reaction states: DNA/RNA-templated elongation, the de novo initiation of RNA synthesis, the first step of nucleotide condensation during de novo initiation and initial NTP loading.

May 1, 2023 · Small RNA molecules have been found to be specifically associated with posttranscriptional gene silencing (PTGS) in both plants and animals. Here, we find that small sense and antisense RNAs are also involved in PTGS in Neurospora crassa.