EYFP is a basic (constitutively fluorescent) green/yellow fluorescent protein published in 1996, derived from Aequorea victoria. It is reported to be a very rapidly-maturing weak dimer with high acid sensitivity.

To aid with the discovery and analysis of plant PCD regulators, we have designed a novel cell death assay based on low cytosolic pH as a marker of PCD. The acidification that occurs in the cytosol during plant PCD was monitored by way of the extinction of YFP fluorescence at low pH.

Yellow fluorescent protein (YFP) dyes are a class of fluorescent proteins that emit yellow fluorescence when excited by light in the blue to green range. These dyes are derived from the green fluorescent protein (GFP) family, which was originally isolated from the …

Yellow fluorescent protein (YFP) is a genetic mutant of green fluorescent protein (GFP) originally derived from the jellyfish Aequorea victoria. [1] Its excitation peak is 513 nm and its emission peak is 527 nm. [2]

Yellow Fluorescent Protein (YFP) is a type of fluorescent protein that emits yellow light when excited by a specific wavelength. It is commonly used in biological studies for dual-color fluorescence applications to enhance precision in gene expression measurements. You might find these chapters and articles relevant to this topic.

Jan 1, 2009 · Enhanced yellow fluorescent protein (EYFP) was generated by introducing the following four mutations in to GFP: T203Y, S65G, V68L, and S72A. Together these mutations alter the spectral properties and fluorescence intensity of EYFP as compared to GFP.

Nov 7, 2022 · We solved the crystal structures of hfYFP (to 1.7-Å resolution), a monomeric variant, monomeric hyperfolder YFP (mhYFP) (1.6 Å), and a cysteine-free mGL variant called FOLD6 (1.2 Å), shedding...

May 1, 2021 · Circular permutated YFP was modified to be an a supersensitive and ratiometric pH probe NocPer. Nitric oxide (NO) lowering intracellular pH (pHi) was discovered as a new biological phenomenon. NO-induced inhibition of cytochrome c oxidase is responsible for the decline in pHi.

The collection of specimens illustrated in this section demonstrates the effectiveness of the Nikon YFP HYQ filter combination with a series of cell lines containing a chimeric EYFP plasmid vector that expresses a fluorescent fusion protein targeted at the cell nucleus.

Dec 24, 2010 · Human hepatitis B virus (HBV) induces a strong activation of Nrf2/ARE-regulated genes in vitro and in vivo. This is triggered by the HBV-regulatory proteins (HBx and LHBs) via c-Raf and MEK.