Precision-cut lung slices (PCLS), small, uniform tissue slices generated from animal or human lungs are increasingly recognized and employed as an ex vivo organotypic model. PCLS …

Precision-cut Lung Slices (PCLS) provide valuable insights into lung physiology and pathology, but they do have limitations. Firstly, PCLS represent a static "snapshot" of lung tissue at the …

Mar 12, 2019 · Using Precision Cut Lung Slice imaging (PCLSi), combined with pharmacological inhibitors of the actin-myosin cytoskeleton, we show that epithelial cell migration plays a …

Jun 1, 2024 · Precision Cut Lung Slices (PCLS) have emerged as a sophisticated and physiologically relevant ex vivo model for studying the intricacies of lung diseases, including …

PCLS enable investigators to study complex interactions among different cell types and the extracellular matrix in the native three-dimensional architecture of the lung. PCLS further allow …

PCLSs from healthy and diseased human tissue are a promising tool, which can 1) recapitulate the complexity of the lung’s native environment; 2) enable the study of the complex …

Sep 11, 2023 · In the process of creating precision-cut lung slices (PCLS), agarose infusion and agarose embedding play a crucial role in obtaining healthy and viable slices that closely …

Jul 19, 2019 · This review covers the practicality of precision cut lung slice (PCLS) generation and benefits of this ex vivo culture system in modeling human lung biology and disease pathogenesis.

Mar 23, 2024 · From unraveling the fundamental mechanisms underlying breathing mechanics to deciphering the immunological aspects of lung tissue and unraveling the pathogenesis of lung …

Here, we describe how precision-cut lung slices (PCLS) can be generated, stained with fluorescently-tagged antibodies, and visualized by confocal microscopy to study the location …