In this study, we constructed three different PTK7-specific CAR (PTK7-CAR1/2/3), each comprising a humanized PTK7-specific single-chain variable fragment (scFv), hinge and transmembrane (TM) regions of the human CD8α molecule, 4-1BB intracellular co-stimulatory domain (BB-ICD), and CD3ζ intracellular domain (CD3ζ-ICD) sequence, and then ...

Here, we examine the therapeutic efficacy of Vatalanib in the treatment of ATRT and a potential target for treatment known as Protein Tyrosine Kinase 7 (PTK7), which we found by transcriptome analysis to be upregulated in ATRT tumors.

We developed an anti-PTK7 chimeric antigen receptor (CAR) and find PTK7 CAR T cells specifically target and kill PTK7-expressing neuroblastoma in vitro. In vivo, human/murine binding PTK7 CAR T cells regress aggressive neuroblastoma metastatic mouse models and prolong survival with no toxicity.

Results: PTK7 controls cellular protrusions and, as a result, directional cell motility and metastasis in fibrosarcoma HT1080 cells. Conclusion: Both PTK7 expression and proteolysis contribute to efficient cell motility and metastasis. Significance: PTK7 is a potential diagnostic biomarker with predictive value and a promising drug target in ...

Jul 20, 2023 · We targeted protein tyrosine kinase 7 (PTK7), a prominent cancer marker, and demonstrated aptamer-mediated biotin transfer to specific lysine residues on the extracellular domain of the protein. This allowed for tracking of PTK7 …

Dec 18, 2015 · Among WNT/PCP components, protein tyrosine kinase 7 (PTK7) is a tyrosine kinase receptor with poorly defined functions lacking catalytic activity. Here we show that PTK7 associates with receptor tyrosine kinase-like orphan receptor 2 (ROR2) to form a heterodimeric complex in mammalian cells.

Results: PTK7 and ROR2 form a heterodimeric complex and bind to WNT5A, promoting JNK phosphorylation and regulating expression of paraxial protocadherin. Conclusion: PTK7 and ROR2 promote cell movement in mammalian cells and coordinate cell polarity during morphogenetic movements.

Aug 12, 2021 · Using in vivo xenograft models of lung cancers with different expression levels of PTK7, systemic delivery of PTK7-CAR2 modified T cells significantly prevented tumor growth and prolonged overall survival of mice.

Sep 1, 2019 · PTK7 proteolytic fragment proteins act like full-length PTK7 to support canonical Wnt-signaling by maintaining LRP6 protein levels. Protein Tyrosine Kinase 7 (PTK7) is as a critical regulator of canonical and non-canonical Wnt-signaling during embryonic development and cancer cell formation.

PTK7 serves as a context-dependent signalling switch for the Wnt pathways (particularly in planar cell polarity related functions such as convergent extension and neural crest cell migration) and appears to have similar functions for plexin and Flt-1 pathways. [7]