This study aimed to investigate the effect of 3-phosphoinositide-dependent kinase 1 (PDK1) interference on the levels of fibrosis and proinflammatory factors in lipopolysaccharide (LPS)-induced ALI and discuss the relevant mechanism.

Feb 19, 2016 · Moreover, we discovered that this process is facilitated by repressed Pdk1 expression and no increase in PDK1 protein abundance, as well as significantly lower phosphorylation of PDH compared with hypoxic conditions, illustrating that M(LPS) macrophages sustain an active pyruvate flux through PDH.

Here we show that pyruvate dehydrogenase kinase (PDK), a key regulatory enzyme for glucose metabolism, is a negative regulator of IL-10 production in LPS-stimulated macrophages.

PDK1 restricts the conversion of pyruvate to acetyl-CoA, which enters the TCA cycle and produces NADH, and blocks OXPHOS in mitochondria (39, 40). Recent findings provide substantial evidence of the function of PDK1 in macrophages.

Jun 15, 2015 · In this study, we found that pyruvate dehydrogenase kinase 1 (PDK1), a key regulatory enzyme in glucose metabolism, plays an important role in the differential activation of macrophages. Knockdown of PDK1 diminished M1, whereas …

Feb 19, 2016 · HIF1 abundance can result in the induction of the gene encoding pyruvate dehydrogenase kinase 1 (PDK1), which inhibits pyruvate dehydrogenase (PDH) via phosphorylation. Therefore, it has been speculated that pyruvate oxidation through PDH is decreased in pro-inflammatory macrophages.

LPS exposure reduced cell viability and promoted cell apoptosis and inflammatory response partly by upregulating circ_RASGEF1B. Circ_RASGEF1B bound to miR-146a-5p and miR-146a-5p interference partly overturned circ_RASGEF1B silencing-mediated effects in LPS-induced TCMK-1 …

May 7, 2015 · Our data show that LPS downregulates the cell survival factor PDK1 and induces podocyte apoptosis, and that blocking the TLR pathway with GIT27 may provide a non-nephrotoxic means to prevent the progression of DN.

Feb 20, 2024 · We find that PD-L1 expression is dramatically boosted in LPS-treated macrophages and that inhibition of PI3K-AKT signal by LY29400 results in a significant decrease in LPS-mediated production of PD-L1 (Fig. 8A).

Jan 6, 2023 · After external cohort validation and protein-protein interaction (PPI) network construction, PDK1 was finally identified as a diagnostic marker for OA. The pharmacological properties of BX795-downregulated PDK1 expression inhibited LPS-induced chondrocyte inflammation and apoptosis and rescued OA-dysregulated autophagy.