CHX abolished this elevation of p53, p21, and Bax as well as the cell cycle arrest induced by serum deprivation. The p53 inhibitor pifithrin-alpha protects HepG2 cells against apoptosis induced by serum withdrawal.

May 25, 2021 · We tested the effects of RYL-687 on p53 stability using the protein synthesis inhibitor cycloheximide (CHX). In the absence and presence of CHX (50 µg/mL), p53 was undetectable in H460 cells...

... confirm the suppressive effect of CHX on the tran- scription-independent pathway, we analyzed its effect on the translocation of p53 to mitochondria, a key initial event in this pathway, in...

Jan 21, 2020 · To determine whether MAVS affects p53 stability, WT and MAVS KO cells were treated with the protein synthesis inhibitor cycloheximide (CHX) for various amounts of time. As shown in Figure 3 H, the p53 protein level in control cells decreased by approximately 50% within 2 h after CHX treatment.

Jul 31, 2018 · Pre-treating HeLa cells with DMOG reduced p53 protein half-life assayed by a CHX-chase experiment in HeLa cells (Figure 2C). These data suggest that hydroxylase inhibitors affect p53 protein levels by post-translational targeting for destruction via the proteasome.

Jul 19, 2021 · TP53 is one of the most extensively studied tumor suppressor genes whose multifaceted mechanisms involve apoptosis, ferroptosis, DNA repair, genomic stabilization, and angiogenesis [2]. Thus,...

Jan 14, 2020 · In this study, we report that FBXW7, a substrate recognition component of the SKP1-CUL1-F-box (SCF) E3 ligase, interacts with and targets p53 for polyubiquitination and proteasomal degradation after exposure to ionizing radiation or etoposide.

To test whether CYD19 could affect wild-type p53 protein stability, vehicle- or CYD19-treated MMTV-PyMT cells were cultured in the presence of CHX (100 μg/ml) to block newly protein synthesis, and p53 degradation was examined.

We next investigated the involvement of the transcription-independent function of p53 using an RNA synthesis inhibitor, actinomycin D (ActD), and a protein synthesis inhibitor, cycloheximide (CHX), and found that the apoptosis was suppressed by CHX but not by ActD.

In the current study, we found that serum withdrawal induces phosphorylation and accumulation of p53. CHX decreases total p53, pp53, p21, and Bax levels and blocks cell death . CHX also blocks the G1-S cell cycle arrest induced by serum withdrawal, most probably due to the decrease in p53 and p21 . The p53 inhibitor pifithrin-α protects HepG2 ...