Feb 1, 2011 · Expression of the c-Myc oncoprotein is affected by conserved threonine 58 (T58) and serine 62 (S62) phosphorylation sites that help to regulate c-Myc protein stability, and altered ratios of T58 and S62 phosphorylation have been observed in human cancer.

Aug 1, 2011 · Here we report that increased stability of WT Myc is a prominent mechanism for Myc overexpression in breast cancer and that this is associated with a change in the ratio of phospho-S62 (pS62) and phospho-T58 (pT58) to more closely match mutant Myc that has increased oncogenic activity.

Sep 7, 2020 · In the T58A mutant, MYC is stabilized with constitutive S62 phosphorylation. When challenged with DMBA/TPA-mediated carcinogenesis, MYC T58A mice had accelerated development of papillomas,...

Tumors with stable MYC expression have elevated levels of phospho-S62 (p-S62)-MYC and low levels of phospho-T58 (p-T58)-MYC [6,8]. Mitogenic pathways, such as RAS–MEK–ERK signaling, can increase p-S62 levels and, thereby, increase MYC stability.

In the present study, the effects of the MYC point mutation T58A on the progression of a cellular tumor antigen p53 (p53) −/− human breast cancer cell line was analyzed, and the mechanism of p53-independent MYC-induced apoptosis was investigated. HCC1937 cells were transfected with mutant (T58A) or wild-type (WT) MYC using lentiviral vectors.

Mar 7, 2013 · To examine Myc dependence upon Ras mutations we have generated MMTV regulated Myc and Myc T58A transgenic mice. Expression of the more stable T58A Myc allele resulted in a reduction in KRas-activating mutations.

Jul 6, 2022 · MYC T58A expressing mice developed clonal T-cell lymphomas with 100% penetrance and conditional knock-out of endogenous Myc accelerated this lymphomagenesis.

Aug 15, 2022 · In SG cells, only the c-Myc (T58) protein was stabilized by the DSF-Cu (I and II) complexes. Hence, our findings could provide novel regulatory insights into the phosphorylation-dependent stability of c-Myc in DSF/copper-complex-treated oral squamous cell carcinoma.

Jan 28, 2022 · Fbw7 binds to a phosphorylated Myc degron centered at threonine 58 (T58), and mutations of Fbw7 or T58 impair Myc degradation in cancers. Here, we identify a second Fbw7 phosphodegron at Myc T244 that is required for Myc ubiquitylation and acts in …

Apr 23, 2012 · To examine Myc dependence upon Ras mutations we have generated MMTV regulated Myc and Myc T58A transgenic mice. Expression of the more stable T58A Myc allele resulted in a reduction in...