Mycobacterium tuberculosis (Mtb) is the causative agent of tuberculosis, which kills 1.8 million annually. Mtb RNA polymerase (RNAP) is the target of the first-line antituberculosis drug rifampin (Rif). We report crystal structures of Mtb RNAP, alone and in …

Feb 28, 2024 · By contrast, the Mtb RNA coverage drops significantly between 200 and 500 nucleotides (nt) downstream of TSSs (Figs. 1b and 2a,b). The RNA coverage drop-off tends to occur well before the...

We show by chromatin immunoprecipitation and DNase I footprinting that the chromosomal origin of replication, ori C, and the promoter for the major secreted immunodominant antigen Ag85B, encoded by fbp B, are MtrA targets.

Apr 20, 2017 · Mycobacterium tuberculosis (Mtb) is the causative agent of tuberculosis, which kills 1.8 million annually. Mtb RNA polymerase (RNAP) is the target of the first-line antituberculosis drug rifampin (Rif). We report crystal structures of Mtb RNAP, alone and in complex with Rif, at 3.8-4.4 Å resolution. …

Jun 21, 2024 · In a recent eBiomedicine issue, Nargan et al. 6 multiplexed RNAscope and Ziehl-Neelsen (ZN) staining/immunohistochemistry to detect Mtb mRNA in ante- and post-mortem human tissues and Acid-fast bacilli (AFB)- vis-à-vis non-AFB- producing antigen 85B (Ag85B).

Sep 24, 2024 · To contribute to the foundational understanding of how Mtb and the related non-pathogenic mycobacterial species, Mycobacterium smegmatis (Msm), perform the essential function of RNA synthesis, we performed a series of in vitro transcription experiments to define the unique enzymatic properties of Mtb and Msm RNAPs. In this study, we ...

Mar 26, 2019 · Human tuberculosis disease (TB), caused by Mycobacterium tuberculosis (Mtb), is a complex disease, with a spectrum of outcomes. Genomic, transcriptomic and methylation studies have revealed...

Mar 18, 2025 · The media-only and Mtb-stimulated RNA-seq datasets contributed roughly equally to the list of features used in the Factor 1 enrichment. The largest cluster in the Factor 1 enrichment, Cluster F1-6, consisted of 136 gene sets primarily related to immune function (Supplemental Table 6). This cluster included the GO interferon gamma production ...

2 days ago · The increasing prevalence of multidrug-resistant tuberculosis (MDR-TB) highlights the urgent need for an efficient approach to identify Mycobacterium tuberculosis complex (MTBC) strains resistant to rifampicin (RIF) and isoniazid (INH). In response, we developed a CRISPR-Cas14a MTB RIF/INH platform that can detect the most common mutations associated with RIF and INH resistance. To evaluate ...

Mycobacterium tuberculosis (Mtb) travels from the lungs of one person through the air to the lungs of another and survives multiple stresses en route, including changes in temperature and in concentrations of oxygen, carbon dioxide, hydrogen ions, salts, and organic solutes. Here, we present a genetically tractable model of transmission …