Jul 15, 1998 · In this study, we demonstrated the clear association of HS1 with Grb2, in vitro and in vivo, indicating that HS1 links signaling between Lck and Grb2. The potential physiologic function of HS1 as a linker between Grb2 and Src family tyrosine kinase is …

GRB2 is a ubiquitously expressed adaptor protein required for signaling downstream of multiple receptors. To address the role of GRB2 in receptor-mediated signaling, the expression of GRB2 was suppressed in human CD4+ T cells and its role …

Grb2 is a scaffold protein that functions by mediating protein–protein interactions. However, our coimmunoprecipitation assay could not prove any meaningful association between Grb2 and Lck, suggesting that Grb2 interacts with other molecule(s) that regulate thymocyte development and Lck …

May 15, 2005 · The phosphorylation of the receptor and Grb2 levels of binding in the transfections with Fyn or Lck were very similar. Thus, both kinases phosphorylate efficiently the Y606 of CD229, crucial for Grb2 association.

Here we report the recruitment of Grb2-SOS1 to CD3zeta of the TCR complex after prolonged anti-CD3 (OKT3) stimulation in T cells with Lck knockdown. Grb2 bound to incompletely phosphorylated ITAM1 with the pY-Y configuration in a solid-phase assay, but was excluded by ZAP-70 in the doubly phosphorylated pY-pY conformation.

GRB2, GADS, and GRAP are especially vital for the function of T cells. These proteins control signaling complexes at the T cell antigen receptor and the adaptor protein LAT, where they facilitate the activation, differentiation, and function of T cells.

We found that inactivating the Grb2 (Y633) and Shc (Y657) binding motifs had little or no effect on Lck activation, while inactivating the p85 motif at Y519 induced a small but statistically significant increase.

Here, we show that short interfering RNA-mediated targeting of Lck caused a dramatic reduction in c-Cbl phosphorylation and a general reduction in protein ubiquitination after TCR stimulation. Specifically, this resulted in reduced ubiquitination of the zeta-chain, yet internalization of TCR/CD3 appeared to be normal after receptor engagement.

Jul 15, 2006 · It has been reported that CD28 associates with signaling proteins such as PI3K, Grb2 family adaptor proteins, Grb2 and Gads/Grf40, Tec family protein tyrosine kinases, Itk and Tec, and Lck (2, 3, 4). Among them, PI3K, Grb2, and Gads bind to the YMNM motif in the CD28 cytoplasmic domain.

Feb 16, 2024 · Our analysis reveals aggressive suppression of GRB-2, an adaptor molecule integral for TCR signaling, as a primary immunomodulatory response against S. typhi infection in endemic settings.