Dec 8, 2016 · Based on the protein structures of RPS4 TIR and L6 TIR (Bernoux et al., 2011; Williams et al., 2014), the TIR domain is predicted to be located between residues 20 and 188 (Figure 1A).

NOD-like receptors (NLRs) are central components of the plant immune system. L6 is a Toll/interleukin-1 receptor (TIR) domain-containing NLR from flax (Linum usitatissimum) conferring immunity to the flax rust fungus.

Mar 17, 2011 · In this study, we used the L6 protein as a model to characterize plant TIR domain signaling activity and mode of action. Mutational analysis showed that the TIR domain is dispensable for AvrL567 recognition but is required for induction of defense responses.

Mutations that perturb either the αD/αE or αA/αE interface of the SNC1 TIR, L6 TIR, or RPS4 TIR abrogate this HR, and also appear to attenuate TIR–TIR interactions in solution (although these interactions are weak and transient in the wild-type proteins).

Mar 25, 2025 · These results demonstrate that cell death signaling induced by L6 TIR, RPS4 TIR, and L6MHV can be monitored in both seedling and adult plants, whereas cell death can only be reached in adult plants grown in soil when induced by an overexpression of RPS4 at 21°C.

In this study, we used the L6 protein as a model to characterize plant TIR domain signaling activity and mode of action. Muta-tional analysis showed that the TIR domain is dispensable for AvrL567 recognition but is required for induction of defense responses.

Feb 1, 2011 · In this study, the crystallization, X-ray diffraction analysis and preliminary structure determination of the TIR domain from the flax resistance protein L6 (L6TIR) are reported. Plate-like crystals of L6TIR were obtained using PEG 200 as a …

May 13, 2024 · By using inducible Arabidopsis transgenic lines expressing TIR-containing NLRs (TNLs) or corresponding isolated TIR domains from Arabidopsis RPS4 and flax L6 NLRs, we showed that immune signaling induced downstream of TNL activation is not affected by an elevation of temperature.

Aug 23, 2019 · In agreement, we show that like the SARM1 TIR domain, the TIR domains from plant immune receptors, including L6 (flax; Linum usitatissimum) and RUN1 (grapevine; Muscadinia rotundifolia) also have self-association–dependent NAD + -cleaving enzyme (NADase) activity.

Mar 17, 2011 · In this study, we used the L6 protein as a model to characterize plant TIR domain signaling activity and mode of action. Mutational analysis showed that the TIR domain is dispensable for AvrL567 recognition but is required for induction of defense responses.