Mar 17, 2023 · Dot blot is a simple form of immunoblotting to detect and quantify dsRNAs. This technique can be used to assess different parameters of the immunoblot, such as antibody efficiency, limit of detection, background, and buffer options.

Anti-dsRNA monoclonal antibody J2 is the gold standard for the detection of dsRNA both in cultured cells and fixed paraffin-embedded histological samples. Applications: ELISA, IF, FACS, IHC, IP, Dot Blot, ChIP, affinity purification, immunoelectron microscopy.

Dot blot assays using SCICONS’s Anti-dsRNA monoclonal antibody J2 are a fast and easy method for the detection of dsRNA in both crude in vitro transcription mixes as well as purified mRNA [1-6]. Estimation of the amount of dsRNA impurity is …

Jun 19, 2024 · To confirm the specificity of RNaseIII for dsRNA, we exposed isolated total RNA to recombinant RNaseIII and subsequently detected the remaining dsRNA by J2 dot-blot.

AVIPure reduced dsRNA to levels that were undetectable by J2 dot blot or ELISA, a >270-fold reduction in dsRNA. The immuno-northern blot confirms that mRNA duplexes are completely removed. AVIPure dsRNA removes dsRNA from mRNA feeds as effectively as RP-HPLC or cellulose/ethanol chromatography.

May 24, 2024 · Here we report a dsRNA-specific immuno-northern blot technique that provides a clear picture of the dsRNA size distributions in transcribed RNA. This technique could complement existing dsRNA analytical methods in studies of dsRNA byproduct synthesis, dsRNA removal, and characterization of therapeutic RNA drug substances.

4 days ago · dsRNA (J2) Mouse mAb recognizes dsRNA where the length of the helix is greater than or equal to 40 base pairs (bp). The ability to recognize dsRNA is independent of the sequence and nucleotide composition of the antigen.

J2 antibody is used in dot blots to detect dsRNA from Leishmania RNA virus (LRV) in the Leishmania parasite L. guyanensis. A serial dilution of 1000 to 10 parasites from LRV-positive and negative control strains - Lg4147LRVhigh and LG4147LRVneg respectively - is shown.

Anti-dsRNA monoclonal antibodies are efficient tools for the detection of dsRNA in cell culture and tissues such as FFPE samples1-6 (Fig. 1, Tab. 1) e.g. for characterization & detection of viruses with dsRNA genomes or intermediates (including SARS, Hepatitis C, Dengue or West Nile Virus).

Oct 10, 2023 · The Anti-dsRNA monoclonal antibody J2 was observed to recognise double-stranded RNA (dsRNA) provided that the length of the helix is greater than or equal to 40 bp.