Aug 30, 2024 · Using bulk-RNAseq of IRF3-2D brain myeloid cells, we identified Z-DNA binding protein-1 (ZBP1) as a target of IRF3 that is relevant across various neuroinflammatory disorders. Lastly, we show IRF3 phosphorylation and IRF3-dependent ZBP1 induction in response to Aβ in primary microglia cultures.

Aug 9, 2024 · IRF3, a critical component of innate antiviral immune responses, interacts with pro-inflammatory transcription factor NF-κB, and inhibits its activity. This mechanism helps suppress inflammatory gene expression in virus-infected cells and mice.

Mar 21, 2025 · IF analysis revealed an increase in the mean fluorescence intensity (MFI) of STING and MAVS along with a significant increase of their co-localization in DNA-PKi-treated PBMC-derived immune cells (Figure 3A).

Sep 30, 2024 · Alveolar macrophages that previously experienced polyIC showed significantly increased levels of nuclear IRF3 upon restimulation with polyIC (polyIC/polyIC) compared with control-experienced cells (control/polyIC) (Figure 5A).

1 day ago · IL-10 is a key suppressor of innate immune activation and inflammatory responses. A well-recognized mechanism of IL-10 action is the suppression of nuclear factor-κB (NF-κB) target genes, such ...

Aug 31, 2023 · Confocal microscopy showed that more IRF3 translocated to the nucleus with overexpressing OTUD6B upon viral infection as measured using mean fluorescence intensity (MFI), the MFI value changed from 8,965 to 11,260 (Fig. 4E and F).

Jan 15, 2009 · In comparison with human influenza (H1N1), the H5N1 virus more strongly activated IFN regulatory factor 3 (IRF3). IRF3 knockdown and p38 kinase inhibition separately and in combination led to a substantial reduction of IFN-β, IFN-λ1, and MCP-1 but only to a partial reduction of TNF-α.

Aug 1, 2016 · Here, we determined that IRF3 expression is upregulated in the adipocytes of obese mice and humans. Signaling through TLR3 and TLR4, which lie upstream of IRF3, induced insulin resistance in murine adipocytes, while IRF3 knockdown prevented insulin resistance.

Oct 9, 2024 · In mammals, nine IRF members have been reported, 10 designated as IRF1 (also known as MAR), IRF2, IRF3, IRF4 (known alternatively as LSIRF/ICSAT/ PIP), IRF5, IRF6, IRF7, IRF8 (also termed as...

For this study, we generated conditional Irf3 -deficient mice while leaving the Bcl2l12 gene intact. Conditional ablation of IRF3 in myeloid cells showed increased survival when challenged with endotoxin-induced shock, indicating a myeloid cell-specific role of IRF3.