Incubation (24 h) of HuH7 cells with LNP Luc mRNA formulations (0.3 μg mRNA/mL) containing 40 mol % ESM resulted in a 100-fold increase in luciferase expression compared with the 10 mol % DSPC formulation.

Jul 6, 2023 · We find that the most efficacious LNP-corona complexes were enriched with high-density lipoprotein (HDL) and, compared to the commonly used corona-biomarker Apolipoprotein E, corona HDL content...

the most potent LNP formulation tested in A549 cells (Figure 3) and induced slightly greater luciferase activity at the highest concentration tested in Huh7 cells. All LNP formulations tested induced significant luciferase expression in both Huh7 and A549 cells with minimal differences at lower concentrations. In vitro expression of mRNA-LNPs A. B.

Feb 16, 2021 · We have developed a high-throughput imaging-based endosomal escape assay utilising a Galectin-9 reporter and fluorescently labelled mRNA to probe correlations between nanoparticle-mediated uptake,...

Jun 16, 2023 · LNP作为已获上市产品验证的有效、安全递送系统，已被广泛开发用于治疗诸如癌症、动脉粥样硬化、心力衰竭和罕见病等疾病的核酸类药物。 上市产品中使用的LNP通常由可电离的脂质/辅助脂质/胆固醇/PEG脂质4组分组成，其摩尔比为50/10/38.5/1.5，也被称为“Onpattro”配方。 Onpattro是由Alnylam开发的世界第一款临床批准用于治疗转甲状腺素诱导淀粉样变性神经疾病的siRNA纳米药物，也是第一款临床获批的LNP递送药物。 配方中的中性辅助脂质常使用二硬 …

Apr 1, 2024 · We describe LNP delivery as a sequence of stochastic transfer processes and review a mathematical model of subsequent protein translation from mRNA. We compile probabilities and numbers obtained from time resolved microscopy.

Lipid nanoparticles (LNPs) have emerged as potent carriers for mRNA delivery, but several challenges remain before this approach can offer broad clinical translation of mRNA therapeutics.

Dec 1, 2020 · Here we present an ionizable lipid nanoparticle (iLNP) based on iBL0713 lipid for in vitro and in vivo expression of desired proteins using codon-optimized mRNAs. mRNAs encoding luciferase or erythropoietin (EPO) were prepared by in vitro transcription and formulated with proposed iLNP, to form iLP171/mRNA formulations.

Our results indicate a fast up-take of BNT162b2 into human liver cell line Huh7, leading to changes in LINE-1 expression and distribution. We also show that BNT162b2 mRNA is reverse transcribed intracellularly into DNA in as fast as 6 h upon BNT162b2 exposure. Keywords: COVID-19 mRNA vaccine, BNT162b2, liver, reverse transcription, LINE-1, Huh7. 1.

In this study, we explored an endogenously LN-targeting lipid nanoparticle (LNP) without the modification of any active targeting ligands for developing an mRNA cancer vaccine.