It is designed for purifying and concentrating femtomoles to picomoles of proteins and peptides, providing improved data quality. containing strong cation exchange resin for the concentration of peptides and removal of salts and nonionic detergents from …

The ZipTip® pipette tip is a 10 μL pipette tip with a bed of chromatography media fixed at its end. It is ideal for concentrating and puriying peptides, proteins or oligo-nucleotides prior to mass spectrometry,HPLC, capillary electrophoresis, and other analytical techniques.

Aspirate and dispense eluant through ZipTip at least 3 times without introducing air. Sample recovery can be improved by increasing elution volume to 10 µL (but at expense of concentration).

ZipTip ® micropipette tips are 10 μL pipette tips with a microvolume bed of chromatography medium fixed at the end. They are designed for purifying and concentrating femtomole to picomole amounts of proteins and peptides, oligonucleotides, and polymers, providing improved mass spec data.

1) Set a P10 pipettor to 10 l, place ZipTip on P10 pipettor Important: Pipet slowly to avoid introducing air into the packing material and to maximize binding; this is very

Dec 24, 2015 · Zwitterionic hydrophilic interaction chromatography (ZIC-HILIC) solid-phase extraction (SPE) combined with direct-infusion nanoESI mass spectrometry (MS) and tandem MS/MS is a well-suited method for the analysis of protein N-glycosylation.

As it would be in the case of Reversed-Phase HPLC, an important precaution to take during the procedure described below is never to allow the Zip-Tip column to dry out during any one of the steps.

Hydrophilic interaction liquid chromatography (HILIC) is arguably the most successful approach for the retention and separation of polar compounds. This technique can be described as a variation of reversed phase chromatography performed using a polar stationary phase.

HILIC-UV/FLD method was developed and glycan identification was performed with LC-MS experiments. HILIC, paired with UV or fluorescence detection, provides. (e.g. glycoform stability studies)—with retention times as qualitative and peak area as quantitative information.

Dec 1, 2016 · Here, we present a novel, simplified technique for HILIC enrichment termed “Drop-HILIC”, which was applied to systematically evaluate the mobile phase effect on ZIC-HILIC (zwitterionic type of hydrophilic interaction chromatography) glycopeptide enrichment.