To ascertain the median lethal dose (LD50) of the influenza A/Aichi/2/1968 virus (H3N2) in mice (n = 10 in each group), the mice were randomly categorized into eight groups.

Feb 18, 2021 · When challenged with rgH9N2 at 20 LD 50 dose, rgH3N2 4xM2e primed mice showed better protection as evidenced by significantly preventing weight loss (average 11% day 6) and enhancing survival...

In contrast, x31 is an H3N2 influenza virus that causes mild to moderate illness in mice, despite containing the six internal genes of PR8. Mice were infected with 10 4 EID 50 of PR8 or x31 and were assessed for illness and mortality.

In this model, an H3N2 reassortant virus with avian virus internal protein genes exhibited efficient replication but inefficient transmission, whereas H5N1 reassortant viruses with four or six human virus internal protein genes exhibited reduced replication and no transmission.

Dec 8, 2022 · This study found the physical RFA could significantly enhance seasonal influenza vaccine-induced immune responses against each viral strain and robustly enhance low-dose (nanograms) H3N2 vaccine...

Jun 23, 2022 · Here, we utilize a computational design strategy to produce epitope-optimized, broadly cross-reactive H3 hemagglutinins in order to create a universal H3N2 influenza vaccine. The Epigraph...

Download scientific diagram | Determination of median lethal dose (LD50) in H3N2 influenza-infected mice. from publication: Investigating the Effects of Laggera pterodonta on H3N2-Induced ...

Sep 13, 2023 · Six weeks after the second boost, mice were bled and challenged with 5× LD 50 of the heterologous A/Philippines/2/1982 (H3N2, X-79) virus. This vaccination experiment was performed in two sets of independent mice (n = 5 mice per set) for serum analysis.

Dec 1, 2014 · Chimeric virosome vaccine efficacy in mice following lethal challenge with PR8 (a) or X47 (b) viruses. At 21 days after the vaccine injection, mice were challenged with five lethal …

In toxicology, the median lethal dose, LD50 (abbreviation for " lethal dose, 50%"), LC50 (lethal concentration, 50%) or LCt50 is a toxic unit that measures the lethal dose of a given substance. [1] The value of LD 50 for a substance is the dose required to kill half the members of a tested population after a specified test duration.