030613 C57BL/6J-Tg (CAG-ARL13B/mCherry/GECO1.2)1Clph/J <em>Arl13b-mCherry-GECO1.2^tg</em> transgenic mice express a ratiometric calcium indicator in all primary cilia that is useful for <em>in vivo</em>/<em>in vitro</em> assessment of Ca²⁺ signaling.

Optical imaging of calcium dynamics using genetically encoded calcium indicators (GECIs) is a powerful tool for systems neuroscience. The current state-of-the-art GECIs emit green light (green GECIs) and are derived from Green Fluorescent Protein (GFP).

Jan 21, 2019 · We report an intensiometric, near-infrared fluorescent, genetically encoded calcium ion (Ca 2+) indicator (GECI) with excitation and emission maxima at 678 and 704 nm, respectively.

In this review, we discuss the developmental history as well as the pros and cons of ion indicators, focusing on genetically encoded calcium indicators (GECIs). Along with GECI properties, we discuss the imaging methods and corresponding GECI signal analysis.

Mar 24, 2016 · We characterized the performance of the new red GECIs in cultured neurons and in mouse, Drosophila, zebrafish and C. elegans in vivo. Red GECIs facilitate deep-tissue imaging, dual-color imaging together with GFP-based reporters, and the use of optogenetics in combination with calcium imaging.

Oct 11, 2018 · Here we present the generation and characterization of transgenic mice expressing the sensitive red GECI jRGECO1a, driven by the Thy1 promoter. Four transgenic lines with different expression patterns showed sufficiently high expression for cellular in vivo imaging.

Imaging calcium transients associated with neuronal activity has yielded important insights into neural physiology. Genetically encoded calcium indicators (GECIs) offer conspicuous potential advantages for this purpose, including exquisite targeting.

Nov 15, 2014 · We now report GCaMP-type low-affinity red fluorescent genetically encoded Ca2+ indicators for optical imaging (LAR-GECO), engineered to have Kd values of 24 μM (LAR-GECO1) and 12 μM (LAR-GECO1.2).

Nov 2, 2017 · Here we report the development, structure, and validation of a new red fluorescent Ca 2+ indicator, K-GECO1, based on a circularly permutated RFP derived from the sea anemone Entacmaea quadricolor.

Mar 1, 2022 · We also describe a novel Calcium DoubleSpy mouse model that conditionally expresses both RCaMP in the cytoplasm and GEM-GECO1 in the mitochondrial matrix, allowing the study of mitochondrial Ca 2+ related physiology and pathogenesis simultaneously in two distinct intracellular compartments. 1.