Guanine nucleotide exchange factor - Wikipedia
Guanine nucleotide exchange factors (GEFs) are proteins or protein domains involved in the activation of small GTPases. Small GTPases act as molecular switches in intracellular …
GEFs and GAPs: Critical Elements in the Control of Small G …
Jun 1, 2007 · Guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs) regulate the activity of small guanine nucleotide-binding (G) proteins to control cellular …
28.5: Small G proteins, GAPs and GEFs - Biology LibreTexts
Explain the roles of GTPase-activating proteins (GAPs) in enhancing GTP hydrolysis and thereby turning off small G protein signaling. Describe how guanine nucleotide exchange factors …
In vitro fluorescence assay to measure GDP/GTP exchange of …
Guanine nucleotide exchange factors (GEFs) are enzymes that promote the activation of GTPases through GTP loading. Whole exome sequencing has identified rare variants in GEFs …
GEF means go: turning on RHO GTPases with guanine nucleotide …
Feb 1, 2005 · Rho GTPases are activated by the direct engagement of guanine nucleotide-exchange factors (GEFs), which catalyse the ejection of GTPase-bound GDP and the loading …
Activation of G Proteins by Guanine Nucleotide Exchange …
Mar 17, 2016 · Most G proteins bind guanine nucleotides (GDP, GTP) in a strongly conserved nucleotide binding pocket—an ancient mechanism preserved in both eukaryotes and …
GEFs: master regulators of G-protein activation - Cell Press
By catalyzing the release of GDP from, and the subsequent uptake of GTP by, G proteins, GEFs enable G proteins to activate downstream effectors such as kinases, adenylate cyclases and …
A pull-down procedure for the identification of unknown GEFs for …
Feb 26, 2016 · After washing and removal of unbound proteins, the GEF can be specifically eluted by addition of GDP or GTP followed by subsequent identification via mass spectrometry.
Synaptic GAP and GEF Complexes Cluster Proteins Essential for GTP ...
Jul 13, 2017 · GTPase-activating proteins (GAPs) and guanine exchange factors (GEFs) play essential roles in regulating the activity of small GTPases. Several GAPs and GEFs have been …
Structural Insights into the Regulation Mechanism of Small …
GEFs promote GDP dissociation from small GTPases to allow for GTP binding, which results in a conformational change of two highly flexible segments, called switch I and switch II, that …