3 days ago · GAPDH (14C10) Rabbit mAb detects endogenous levels of total GAPDH protein. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys260 of human GAPDH protein. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation …

To accurately determine protein expression and interpret Western blot results, it is important to use loading controls. A loading control antibody helps determine if samples have been loaded equally across all wells and confirms effective protein transfer during the western blot protocol.

Antibodies that detect GAPDH can be used in several scientific applications, including Western Blot, Immunohistochemistry, Immunocytochemistry, ELISA and Immunoprecipitation. These antibodies target GAPDH in Human, Mouse, Rat, Rabbit and Yeast samples.

Apr 1, 2012 · Western blot analysis of the endogenously expressed GAPDH in prokaryotes (E. coli) and eukaryotes and protein stability under several different treatments probed by the mouse-derived anti-GAPDH monoclonal antibody.

GAPDH is a popular loading control for Western blot assays, highly expressed in most cells, with an essential role in the glycolytic pathway and DNA replication.

Housekeeping proteins (HKPs), such as β-actin, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and β-tubulin, are commonly used to normalize protein expression. But HKP expression can be impacted by certain experimental conditions, such …

GAPDH, a heme chaperone, has been previously implicated in the incorporation of heme into iNOS and soluble guanylyl cyclase (sGC). Since sGC is critical for myoglobin (Mb) heme-maturation, we investigated the role of GAPDH in the maturation of this globin, as well as hemoglobins α, β, and γ.

Nov 9, 2011 · Find out why loading controls like GAPDH and Beta-Actin are imperative to accurately determine protein expression and correctly interpret Western blot results.

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is a well-known housekeeping gene with functions in glycolysis. Many biologists are familiar with the gene and use GAPDH antibodies for a loading control when performing western blots.