EMD-2833 - Cryo-EM structure of TMV reconstructed from micrographs recorded with the K2 Summit DED

Sep 1, 2013 · Diffraction and cryo-EM provide a detailed picture of TMV in various states, even during assembly. TMV is surprisingly resilient against many polar organic solvents, and against protonation. A large range of macro- and nanoscale spectroscopic methods can be used to discover more physical properties.

Here, we present two cryo‐EM structures of the helical TMV assembly at 2.0 and 1.9 Å resolution in conditions of high Ca 2+ concentration at low pH and in water.

Here, we present two cryo-EM structures of the helical TMV assembly at 2.0 and 1.9 Å resolution in conditions of high Ca 2+ concentration at low pH and in water.

Feb 1, 2015 · Here, we provide a comparative case study of highly ordered TMV recorded on different direct electron detectors to establish recording and processing conditions that enable structure determination up to 3.2 Å in resolution using cryo-EM. 1. Introduction.

Jan 1, 2018 · TMV and TMV-like particles have been coupled to a number of organic molecules, including chromophores, polymers, or certain heavy metal complexes, to create classes of TMV-derived effector colloids. Such hybrid objects are envisaged for biomedical applications inside living organisms.

In this study we used TMV to test whether a near atomic resolution structure can be obtained using a commercially available 4k × 4k CCD camera with an ultra sensitive scintillator to collect cryo-EM images at 300 keV. TMV is a highly ordered plant virus.

EMPIAR, the Electron Microscopy Public Image Archive centered at EMBL-EBI, is a public resource for raw electron microscopy images related to EMDB, contains micrographs, particle sets and tilt-series.

Here we present a structural analysis of TMV as a test object to assess the quality of cryo-EM images recorded at 300 keV on a CCD camera. The 4.6A TMV structure obtained is consistent with the previous cryo-EM structure and confirms that there is a second helical form of TMV.

In combination with confocal laser scanning microscopy, EM enables live imaging of cells and tissues with high-resolution analysis. Here, we describe the pivotal role played by EM in the study of viruses, from structural analysis to the biological relevance of the viral metagenome (virome).