Differential scanning calorimetry (DSC) is a thermoanalytical technique in which the difference in the amount of heat required to increase the temperature of a sample and reference is measured as a function of temperature. [1] Both the sample and reference are maintained at nearly the same temperature throughout the experiment.

Differential scanning calorimetry (DSC) is an analytical technique that measures the molar heat capacity of samples as a function of temperature. In the case of protein samples, DSC profiles provide information about thermal stability, and to some ...

Nov 17, 2015 · We can extract multiple thermodynamic parameters from DSC data, such as ΔH, ΔH vH, ΔCp, and ΔG … but the most widely used parameter is T m. Incidentally this is also the easiest and most accurate value to obtain – it is simply the temperature at the peak maximum.

DSC is the most direct and sensitive approach for characterizing the thermodynamic parameters controlling noncovalent bond formation (and theref ore stability) in proteins and other macromolecules. In an experiment requiring only a few micrograms of material, the protein is thermally unfolded,

Mar 1, 2013 · DSC is one of main techniques used to assess thermal stability of formulations, often in combination with DSF and other analytical methods using temperature, with the apparent Tm obtained being a key indicator of desirable shelf life.

In the case of polymers, the peak temperature, T m, is a measure of the average melting temperature of the crystallites. With second order phase transitions, T m is the characteristic temperature of the transition, and with mixtures, the peak maximum defines the liquidus curve in the phase diagram.

Differential Scanning Calorimetry (DSC) is an analysis technique used to characterize the stability of a protein or other biomolecule directly in its native form. It does this by measuring the heat change associated with the molecule’s thermal denaturation when heated at a constant rate.

Nov 30, 2022 · Temperature Modulated DSC (TM-DSC) is a technique that combines periodic temperature control performed by AC calorimetry, a technique that measures the specific heat capacity of a sample, and constant rate temperature control performed by DSC measurement.

DSC is a biophysical characterization technique used to study the stability of compounds including lipids, proteins, and oligonucleotides, in their native state.

TM from DSC is a parameter which is frequently used to rank-order stability in candidate selection (developability), formulation screening, and process development.