Jul 15, 2020 · Here, we describe the design, fabrication, purification, characterization and potential biomedical applications of a self-assembling tetrahedral DNA nanostructure (TDN)–based multifunctional...

Apr 1, 2025 · The tetrahedral DNA nanostructure (TDN) stands out as a widely utilized nanostructure, attracting attention for its high biostability, excellent biocompatibility, and versatile modification sites. The capability of DNA tetrahedron to interact with various signal outputs makes it ideal for developing functional DNA nanostructures in biosensing ...

Dec 7, 2021 · Tetrahedral DNA nanostructure (TDN) is a pyramidal three-dimensional nanostructure formed by the complementary pairing of four single-stranded DNA [14, 18]. TDN has been proposed as promising drug carriers due to their high stability, biocompatibility, rich functional modification sites, suitability for different drugs and excellent cellular ...

Apr 1, 2019 · Among these tetrahedral DNA nanostructures (TDNs), as typical multi-arm DNA tiles for 3D polyhedral, is a major research focus owing to their structural and biological features. TDNs have yielded satisfactory results as biological regulators, …

Jul 24, 2024 · Multiple precisely designed single-strand DNAs can be fabricated into complex, three-dimensional DNA nanostructures through a simple self-assembly process. Among all of the synthetic DNA nanostructures, tetrahedral DNA nanostructures (TDNs) stand out as the most promising biomedical nanomaterial.

Jan 20, 2023 · Owing to the excellent biocompatible nature, programmability and remarkable cell and tissue permeability, the tetrahedral DNA nanostructure (TDN) has proven its value in the delivery of various...

Jun 26, 2020 · A tetrahedral DNA nanostructure (TDN), the most popular DNA nanostructure, with high stability and simple synthesis procedure, is a promising candidate as nanocarriers in drug delivery and bioimaging platforms, particularly in precision …

Feb 1, 2024 · In this study, the interaction of tetrahedral DNA nanostructures (TDNs) and double-stranded DNA (dsDNA) with DNase I is investigated using all-atom molecular dynamics simulations. Our results indicate that DNase I can effectively bind to all dsDNA molecules, and certain key residues strongly interact with the nucleic bases of DNA.

Apr 15, 2025 · For TDN assembly analysis, 0.6% agarose gel electrophoresis was used in 1x TBE buffer at a constant voltage of 60 V for 2 h. Assembly of TDN-AuNPs. First, DNA-AuNPs were prepared by the freezing-assisted method. The received thiolated DNA samples were directly dissolved in Milli-Q water without further treatment.

Nov 20, 2019 · Herein, the dynamic process of transporting single tetrahedral DNA nanostructures (TDNs) is monitored in real time using a force-tracing technique based on atomic force microscopy. The results show that transporting single TDNs into living HeLa cells need ≈53 pN force and ≈25 ms duration with the average speed of ≈0.6 µm s −1.