PEG PFP Ester must first be dissolved in a minimal amount of an organic solvent, such as dimethylsulfoxide (DMSO) or dimethylformamide (DMF) and then added to the buffer …
Bioconjugation Discussion: Reasons for Choosing NHS, TFP, or PFP …
DMF is a bit tricky to use since it can degrade over time, forming free amines that react with the NHS ester. You should always use fresh DMF of the highest grade possible, and you should …
Use of pentafluorophenyl esters for one-pot …
Jul 8, 1996 · Treatment of 4-(thiomethyl) benzoic acids 10 with pentafluorophenyl trifluoroacetate in a mixture of pyridine/DMF provides the thioacyl amide 11 (75% yield, Scheme 3), fiarther …
Efficient DNA–Polymer Coupling in Organic Solvents: A Survey
Dec 16, 2016 · Recently, pentafluorophenyl (PFP) esters have been used for the functionalisation of polymers produced by RAFT polymerisation 41,42,43,44.
Pentafluorophenyl ester activation for the preparation of N,N ...
Mar 24, 1997 · The stability of pre-activated Pfp ester intermediates and the facility with which N,N′-diaroylhydrazines can be synthesized using this procedure (stirring at room temperature …
Dimethylformamide - Wikipedia
Dimethylformamide, DMF is an organic compound with the chemical formula H C O N(CH 3) 2. Its structure is HC(=O)−N(−CH 3) 2. Commonly abbreviated as DMF (although this initialism is …
Biotin-PFP ester - BPS Bioscience
More reactive than the NHS ester group, targeting both primary and secondary amines. Suitable for biotin labeling of both proteins and amino acids. Soluble in DMF or DMSO. Biotinylation …
Synthesis of analogues M01 to M04: (a) PFP-TFA (1.2
Simplified analogues of the potent human amylase inhibitor Montbretin A were synthesised and shown to bind tightly, Ki = 60 and 70 nM, with improved specificity over medically relevant...
Self-quenching polysaccharide-based nanogels of pullulan/folate ...
Aug 1, 2010 · While the nanogels showed photoactive properties including fluorescence and singlet oxygen generation in organic solvent (DMF), these properties were suppressed in PBS …
PFP N™ (Preservative-Free Pump) - Silgan Dispensing Systems
Protecting preservative-free or oxygen sensitive nasal formulations, PFP N sprayers are tested to ensure full protection against microbial contamination.