May 7, 2021 · Loss-of-function of CRWN1 and CRWN2 produces a synergistic effect in activating PR1. In 10-day-old crwn1 crwn2 double-mutant seedlings, PR1 was expressed approximately 35 times as high as in WT plants ( Figure 4E ).

May 7, 2024 · Because defects in seeds often stem from abnormal endosperm development, we focused on crwn1 crwn2 (crwn1/2) endosperm. These mutant seeds exhibited enlarged chalazal endosperm cysts and increased expression of stress-related genes and the MADS-box transcription factor PHERES1 and its targets.

The CRWN1 C-terminus interacts with NAC WITH TRANSMEMBRANE MOTIF1-LIKE9 (NTL9), a transcription factor known to be involved in repressing PATHOGENESIS-RELATED1 (PR1) . The presence of CRWN1 increases the DNA-binding activities of NTL9, indicating CRWN1 plays a specific role in PR1 regulation .

Oct 9, 2017 · In this article we show that crwn1 crwn2 double mutant displays an enhanced resistance against virulent bacterial pathogens, and both virulent bacteria and salicylic acid (SA) induce transcription of CRWN1 gene as well as proteasome-mediated degradation of …

We demonstrate that CRWN1 and CRWN2 physically associate with the DNA damage repair proteins RAD51D and SUPPRESSOR OF NPR1-1 Inducible 1 (SNI1) and act in the same genetic pathway to mediate this process. Moreover, CRWN1 and CRWN2 partially localize at γ-H2AX foci upon DNA damage.

In this article we show that crwn1 crwn2 double mutant dis-plays an enhanced resistance against virulent bacterial pathogens, and both virulent bacteria and salicylic acid (SA) induce transcription of CRWN1 gene as well as proteasome-mediated degradation of CRWN1 pro-tein.

CRWN1 and CRWN2, acting redundantly, are required for nor-mal levels of H3K27me3 deposition on the PR1 locus. We observed a similar pattern when examining H3K27me3 levels associated with the AGAMOUS (AG) locus, which is not known to be regulated by SA. The reduction in H3K27me3 at the PR1 locus in crwn1 crwn2 mutants might explain the elevated ...

Oct 3, 2023 · Quadruple crwn null genotypes were rarely transmitted through gametophytes. We focused on the crwn1 crwn2 (crwn1/2) endosperm, which exhibited enlarged chalazal cysts and increased expression of stress-related genes and the …

Jan 29, 2019 · Loss of CRWN proteins induces the expression of the salicylic acid biosynthetic gene ISOCHORISMATE SYNTHASE1, which leads to spontaneous defense responses in crwn1 crwn2 and crwn1 crwn4 mutants, which are deficient in two of the four CRWN paralogs.

Loss-of-function of CRWN1 and CRWN2 produces a synergistic effect in activating PR1. In 10-day-old crwn1 crwn2 double-mutant seedlings, PR1 was expressed approximately 35 times as high as in WT plants ( Figure 4E ).