Jan 1, 2020 · The Cellular Thermal Shift Assay (CETSA®) is well suited for identifying target engagement between ligands and their protein targets. Several studies have shown that CETSA combined with MS is a powerful technique that allows unlabeled target deconvolution in complex samples such as intact cells and tissues in addition to cell lysates and other ...

Apr 27, 2020 · We recently demonstrated that the cellular thermal shift assay (CETSA) protocol in its two variants: the melt curve and the isothermal dose-response, represents a comprehensive strategy for...

Mar 5, 2024 · Purpose: This study aimed to profile protein targets of quercetin at the proteome level. Methods: A label-free CETSA-MS proteomics technique was employed for target enrichment and identification. The R package Inflect was …

cetsa in cancer cell biology. With the current high resolution MS-CETSA, it is possible to monitor global changes of protein interaction states during specific steps of cancer progression, and in turn identify potentially important molecular processes that can be pharmacological targets.

Aug 7, 2014 · The cellular thermal shift assay–CETSA–exploits this to characterize intracellular drug-protein engagement. Thermal shift assays are used to study thermal stabilization of proteins upon...

This study aims to assess these host cell targets for a panel of FDA-approved antiviral compounds including remdesivir, using the cellular thermal shift assay (CETSA) coupled with mass spectrometry (CETSA MS) in noninfected cells.

Jan 1, 2020 · Several studies have shown that CETSA combined with MS is a powerful technique that allows unlabeled target deconvolution in complex samples such as intact cells and tissues in addition to cell lysates and other protein suspensions.

Jul 25, 2023 · Three formats of CETSA for NP-based drug development: classic Western blotting (WB)-CETSA for target validation, thermal proteome profiling (TPP, also known as MS-CETSA) for unbiased proteome-wide target identification, and …

Feb 8, 2025 · Cellular Thermal Shift Assay (CETSA) is a valuable technique for studying protein stability and interactions within cells. It plays a crucial role in drug discovery by validating potential drug targets through the observation of protein denaturation under varying thermal conditions.

Apr 6, 2023 · Mass spectrometry-based thermal stability assays (MS-TSA) are a promising approach to characterize protein-ligand interaction, and several strategies have been recently developed to...