Nov 16, 2021 · Here we directly measure information flow from receptors to Cdc42 by pairing zebrafish parapinopsina, an optogenetic G Protein Coupled Receptor with reversible ON/OFF control, with a spectrally...

Jan 11, 2022 · In this study, we perform ISC-specific deletion of CDC42 by Olfm4-CreER, which induces intestinal hyperplasia and loss of polarity, mimicking whole intestinal epithelium loss-of-function effects. Surprisingly, in the Olfm4-CreER CDC42 knockout (KO) mice, the ISC population is severely diminished, and the TA cell population is expanded.

Aug 17, 2021 · Here, we report that Cdc42 is essential for germline niche development. Sertoli cell-specific Cdc42 -deficient mice showed normal premeiotic spermatogenesis. However, germ cells gradually disappeared during haploid cell formation …

Cdc42 knockout (KO) is embryonic lethal in mice, with the affected animals dying before embryonic day 7.5; thus, tissue-specific Cdc42 KO models have been developed to study Cdc42 function beyond early embryogenesis 15, 16.

Aug 6, 2024 · Cdc42 activity in the Cftr KO and WT enteroids could be reduced by inhibition of the Wnt transducer Disheveled. With the use of a dye permeability assay, Cftr KO enteroids exhibited increased paracellular permeability to 3 kDa dextran relative to WT.

Jan 11, 2022 · We show that in CDC42 ISC-KO crypts, Scribble binding to the MST1/2-Lats1/2-YAP complex is reduced, leading to reduced phosphorylation of MST1/2, Lats1/2, and YAP, reduced degradation of YAP, and increased nuclear localization of YAP.

Nov 13, 2009 · To address these fundamental questions, we have studied the functional role of Cdc42 in pancreatic tubulogenesis. We present evidence that Cdc42 is essential for tube formation, specifically for initiating microlumen formation and …

Nov 9, 2015 · Proteome analyses indicated that Cdc42 knockout (ko) DCs only inefficiently degrade the MHCII-associated invariant chain chaperone (CD74, or Ii), a defect that was mimicked by treating wild-type (wt) DCs with actin inhibitors.

Oct 1, 2013 · Cdc42 regulates proliferation, polarity, migration, and differentiation of intestinal epithelial cells in mice and maintains intestine epithelial barrier and homeostasis. Defects in Cdc42 signaling could be associated with microvillus inclusion disease. See Covering the Cover synopsis on page 700.

Jan 11, 2022 · Inducible ablation of Scribble in intestinal epithelial cells mimics that of CDC42 KO defects, including crypt hyperplasia and Hippo signaling activation. Mammalian epithelial polarity regulates ISC/TA cell fate and proliferation via a Hippo-Ereg-mTOR cascade.