Dec 12, 2012 · The CD11b + Ly6C ++ Ly6G-population had expanded extensively in the spleen of granuloma-carrying mice. In contrast, mainly the CD11b + Ly6C + G + population, and to a lower extent the CD11b + Ly6C ++ Ly6G-population, expanded in spleen of mice with experimental autoimmune encephalomyelitis (EAE).

On CD11b+ cells, they are expressed on Ly6C (hi) monocytes, neutrophils, and eosinophils (to start with). Here are my recommendations for gating these guys, so we can find out what it is: 1. Gate...

Dec 24, 2019 · Collectively, our findings suggest that CD11b + Ly6G-Ly6C-MDCs could be manipulated to enhance the efficacy of chemotherapy for brain tumors. However, our study also cautions that the timing of any MDC manipulation may be critical to …

We found that CD11b + Ly6C + Ly6G + cells produce Type I interferons and large quantities of reactive oxygen species. We also observed that depletion of Ly6G + cells results in a dramatic increase in tissue damage at the site of infection.

CD11b + Ly6C high (Ly6C hi) cells (inflammatory monocytes) and CD11b + Ly6C low cells (reparative monocytes) were recruited into the liver following I/R injury. Treatment with clodronate-loaded liposomes, which transiently deplete systemic macrophages, alleviated hepatic damage.

Nov 4, 2015 · Phenotypic characterization of these CD11b + Ly6C ++ Ly6G – cells by flow cytometry and RNA-Seq revealed an activated phenotype showing both pro- and anti-inflammatory features, including the expression of inducible nitric oxide synthase (iNOS) by a fraction of cells in an IFN-γ-dependent manner.

Dec 12, 2012 · Methods: CD11b(+)Ly6C(++) and Ly6G(+) cells were isolated from spleen, tumor tissue or inflammatory granulomas. S100A9, Arginase 1 and iNOS gene expression in the various CD11b(+) cell populations was analyzed using Q-PCR.

Nov 23, 2015 · (B) CD11b + Ly6G − myeloid cells can be separated into three populations based on Ly6C expression, with Ly6C low myeloid cells constituting the bulk of these cells in the naïve animal. CD11b + Ly6G − Ly6C low cells show a biphasic response after CFA injection, peaking at 24 h and again at 14 d, whereas they make up the majority of cells ...

CD11b + Ly6C ++ and Ly6G + cells were isolated from spleen, tumor tissue or inflammatory granulomas. S100A9, Arginase 1 and iNOS gene expression in the various CD11b + cell populations was analyzed using Q-PCR. The suppressive activity of the CD11b + cell populations from different donors was studied in co-culture experiments.

Mechanistically, depletion of CD11b + Ly6G − Ly6C − MDCs blunted therapy-induced increases in tumor-associated macrophages (TAMs) and compromised therapy-elicited angiogenesis. Collectively, our findings suggest that CD11b + Ly6G − Ly6C − MDCs could be manipulated to enhance the efficacy of chemotherapy for brain tumors. However, our ...