Bone marrow derived macrophages (BMDM) present an ideal in vitro model to understand the mechanisms controlling polarization of activated macrophages 15. Specifically, activation of M1 macrophages can be induced by lipopolysaccharides (LPS) stimulation, while polarization of M2 macrophages can be induced by IL-4 and/or IL-13.

Jan 21, 2020 · Here, the effects of cyH on human THP-1 macrophages and murine bone marrow-derived macrophages (BMDM), either unpolarized M0, or polarized in M1 or M2 phenotype were studied. In M0...

Dec 23, 2024 · Specifically, resting bone marrow-derived macrophage (BMDM—M0) induces the reactivation of VBNC yeast, while M1 pro-inflammatory BMDM induces fewer reactivation events . In line with differential responses to dormant Cn, we observed that, unlike active yeast, VBNC Cn enhanced the production of the chemokine ligand (CCL)4 in macrophages

Non-activated quiescent macrophages are referred to as M0 macrophages. Polarization of M0 macrophages towards an M1-like phenotype can be induced by stimulation of naive BMDMs with bacterial lipopolysaccharide (LPS) 9.

当遇到不同的抗原刺激时，单核细胞会变成高度杀伤性的巨噬细胞（M1），或变成免疫抑制性的巨噬细胞（M2）。 为便于各位老师对巨噬细胞功能及外界影响等方面进行体外研究，本文总结了常见的4种不同来源巨噬细胞的体外计划诱导方案。 图1. 巨噬细胞的极化及M1和M2巨噬细胞的特异性功能. 实验前准备. 1. 细胞诱导因子 2. 消化酶3. 无菌PBS4. 培养基、血清5. 流式抗体. 一、 THP-1细胞 的极化. 1) THP-1细胞铺板，将佛波酯 PMA （100 ng/ml）加入完全培养基中，诱导其 …

作者观察到，用细胞因子刺激培养物中的骨髓源性巨噬细胞 (bmdm) 诱导 m1 或 m2 极化，显示出明显不同的细胞形态。添加 lps 和 ifn-γ 会刺激 m1 极化，导致细胞在刺激后 24 小时内变平成圆形、煎饼状。相反，添加刺激 m2 极化的 il-4 和 il-13 会导致细胞伸长（图 1a）。

Jul 18, 2024 · Histograms and bar graphs show the Sptlc2 and ceramide mean fluorescence intensity (MFI) of M0-like, M1-like, and M2-like BMDM (n = 3 biological replicates).

Sep 11, 2022 · We further compared the sera with and without heat inactivation on ADCC activity in the presence of BMDMs (M0) and their absence: BMDM (−).

Here, we describe a procedure to isolate and cultivate macrophages from murine bone marrow that will provide large numbers of macrophages for studying host-pathogen interactions as well as other processes.

Mar 19, 2021 · This protocol describes the collection of bone marrow cells from the femur and tibia of mice, differentiation into bone marrow-derived macrophages (BMDM cells), and sampling from cultures. This protocol focuses on the efficient preparation of BMDM cells, providing a way to assess the function of macrophages.