Oct 23, 2016 · AmtR is a transcriptional repressor of urea metabolism in M. smegmatis. AmtR shows extraordinary, tight DNA binding (picomolar). AmtR expression is regulated through a cis -encoded (trans -acting) small RNA. 3D structure of AmtR (2.0 Å) reveals an overall TetR-like dimeric structure.

Mar 1, 2017 · A structure comparison with the previously solved structure of AmtR in a trigonal crystal system suggests a 6:6 stoichiometric association of three AmtR dimers with two trimeric GlnK complexes that prevents AmtR from binding to DNA and induces gene transcription.

Jul 10, 2011 · In this study, we address the role of conserved and non-conserved binding sites upstream of AmtR target genes and the lacking autoregulation of AmtR although a putative AmtR binding site present upstream of the amtR gene (Jakoby et al., 2000, Muhl et al., 2009).

The TetR family member AmtR is the central regulator of nitrogen starvation response in Corynebacterium glutamicum. While the AmtR regulon was physiologically characterized in great detail up to now, mechanistic questions of AmtR binding were not ...

Nov 1, 2009 · AmtR, a member of the TetR family of transcription regulators, is a global regulator of nitrogen control in Corynebacterium glutamicum. Unlike other TetR-family members, which are regulated by small-molecule effectors, AmtR is regulated by a protein called GlnK.

Jan 18, 2002 · In this study, we investigated regulation of ammonium uptake in Corynebacterium glutamicum, a Gram-positive soil bacterium closely related to Mycobacterium tuberculosis. As shown by Northern blot hybridizations, regulation occurs on the level of transcription upon nitrogen starvation.

Mar 1, 2017 · Here we report the structure and function of the M. smegmatis AmtR and describe the role of AmtR in the regulation of nitrogen metabolism in response to nitrogen availability.

Oct 23, 2016 · Mycobacterium smegmatis encodes for two global nitrogen regulators, GlnR and AmtR. AmtR is a transcriptional repressor of urea metabolism in M. smegmatis. AmtR shows extraordinary, tight DNA binding (picomolar). AmtR expression is regulated through a cis -encoded (trans -acting) small RNA.

Jul 2, 2019 · From the respective standard stock solution, working standard solution was prepared containing 75μg/ml of PRGB and 10μg/ml of AMTR separately in mobile phase (Fig. 3).

Feb 9, 2022 · Real-time quantitative PCR (RT-qPCR) analysis and sfGFP (superfolder green fluorescent protein) analysis reveal that AmtR directly and negatively regulates the transcription and expression of crn A, cre T, csh A, and hyu B. A growth test shows that C. glutamicum ATCC 14067 can use creatine or creatinine as a sole nitrogen source.