Aug 16, 2024 · In our investigation, we systematically examined the expression levels of IFI-16, apoptosis-associated Speck-like protein (ASC), pro-caspase-1, cleaved-caspase-1, gasdermin-D (GSDMD), and cleaved-GSDMD over a time course under hypoxic and nutrient-deprived conditions in AC16 cells.

Feb 21, 2023 · In this study, we reveal the critical transcriptional role of KLF7 in pathological cardiac hypertrophy and cardiac metabolism. We identified the rate-limiting enzyme for glycolysis, PFKL, and a key...

Oct 19, 2023 · Here, we report a novel small molecule HHQ16, an optimized derivative of astragaloside IV, which effectively reversed infarction-induced myocardial remodeling and improved cardiac function by...

Jul 15, 2022 · WGA-FITC staining indicates the size of a single cell of AC16 (x200 scale bar 50 μm; E). The effect of PM 2.5 on the hypertrophic protein expression of ANP, BNP in AC16, and their semi-quantitative level (F & G).

Jul 4, 2024 · Here, we utilised low‐intensity pulsed ultrasound (LIPUS) on MIRI mice model and demonstrated that LIPUS reduced the infarcted area and improved cardiac dysfunction. Additionally, we found that LIPUS alleviated MIRI‐induced mitochondrial dysfunction.

May 18, 2020 · Following CHX treatment for various periods of time, PP2Acα was degraded faster in REGγ-WT NRCMs or AC16 cells than in REGγ-knockdown NRCMs or AC16 cells (Fig. 4i–l).

In AC16, Ad-METTL3 improved the hypoxia-stimulated decrease in myocardial proliferation and increase in apoptosis (Supplementary Fig. 2). In addition, si-METTL3 mimicked the pathological effects of hypoxia in AC16 (Supplementary Fig. 3 ).

By computationally optimizing the rotating magnetic field using COMSOL™ with two-dimensional (2D) finite element analysis (FEA), we built a highly uniform MTS system for remotely stimulating mechanotransduction pathways in AC16 human cardiomyocytes (AC16-hCMs) and human cardiac fibroblasts (hCFs).

Sep 28, 2023 · Using the cardiomyocyte cell lines AC16 and H9c2, we determined that autophagy was initiated during AIC. Our results suggest that inhibition of autophagy at its early phase with SAR405 combined with DEX represents an effective therapeutic strategy to prevent AIC.

May 15, 2024 · Wheat germ agglutinin (WGA) staining was carried out to label the cell membranes. The frozen heart sections were firstly fixed under 4% paraformaldehyde. After blocked, the slides were incubated with WGA-FITC (1:200, GeneTex, USA) …