Accurate and early detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection is critical for minimizing spread and initiating treatment. Among test methods, real-time reverse-transcriptase polymerase-chain-reaction (rRT-PCR) is considered the gold standard.

Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique combining reverse transcription of RNA into DNA (in this context called complementary DNA or cDNA) and amplification of specific DNA targets using polymerase chain reaction (PCR). [1]

Mar 27, 2020 · Real time RT–PCR is a nuclear-derived method for detecting the presence of specific genetic material in any pathogen, including a virus. Originally, the method used radioactive isotope markers to detect targeted genetic materials, but subsequent refining has led to the replacement of isotopic labelling with special markers, most frequently ...

What is the Quest SARS-CoV-2 rRT-PCR? The test is designed to detect the virus that causes COVID-19 in upper and lower respiratory specimens such as nasopharyngeal, oropharyngeal or...

Accurate and early detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection is critical for minimizing spread and initiating treatment. Among test methods, real-time reverse-transcriptase polymerase-chain-reaction (rRT-PCR) is considered the gold standard.

Feb 18, 2022 · Designing a fast and accurate PCR-based assay is of great importance for the early detection of this virus and more efficient control of the spread of this disease. This study describes a fast,...

So far, the real-time reverse transcriptase-polymerase chain reaction (RT-qPCR) is the assay of choice for COVID-19 diagnosis considering its rapidity and accuracy in informing on active coronavirus (CoV) infection.

Fast and accurate, real-time RT–PCR has been critical in detecting, tracking and studying COVID-19. But what is it, and how does it work? Let us explain.

Dec 12, 2021 · Serum samples were tested with two rRT-PCR assays: cobas® SARS-CoV-2 test (cobas® test), a qualitative assay for detection of SARS-CoV-2 RNA; and TaqPath™ COVID-19 CE-IVD RT-PCR Kit (TaqPath™ test), a multiplex RT-PCR assay for qualitative detection of SARS-CoV-2 nucleic acids.

Nov 21, 2023 · Extraction-free rRT-PCR methods aim to simplify and expedite the testing process by bypassing the RNA extraction step, which is time-consuming, labor-intensive.