Explore Bio-Rad’s comprehensive pGLO and GFP teaching resources, including pGLO plasmid maps, hands-on lab activities, and free presentations, videos, and case studies. Learn how to use GFP to illuminate gene regulation and expression in your classroom.

The pGLO plasmid is an engineered plasmid used in biotechnology as a vector for creating genetically modified organisms. The plasmid contains several reporter genes , most notably the green fluorescent protein (GFP) and the ampicillin resistance gene.

The pGLO plasmid contains an origin or replication, a selectable marker, and the gene for Green Fluorescent Protein (GFP). The plasmid also contains a gene for the arabinose C protein, which is a protein that regulates expression from the arabinose BAD promoter (P BAD).

Bacterial transformation plasmid for regulated expression of GFP. Sequence Author: Bio-Rad. Explore Over 2.7k Plasmids: Fluorescent Protein Genes & Plasmids | More Plasmid Sets. Efficient cleavage requires at least two copies of the SgrAI recognition sequence. Sticky ends from different BsmBI sites may not be compatible.

Jan 1, 2019 · A commonly used classroom experiment to demonstrate genetic transformation employs the pGLO plasmid and Escherichia coli as the host. A detailed structure of the plasmid is shown in Figure 1.

•The pGLO plasmid, which contains the GFP gene, also contains the gene for beta-lactamase, which provides resistance to the antibiotic ampicillin, a member of the penicillin family. The beta-lactamase protein is produced and secreted by bacteria that contain the plasmid.

pGLO is a trademarked engineered plasmid widely used in genetic research to create genetically modified organisms (GMOs). This plasmid has been specifically designed to include several key genes that serve as markers for scientists.

plasmid. In this lab, the pGLO plasmid has the GFP gene that codes for the green fluorescent protein and a gene that codes for a protein that gives the bacteria resistance to an antibiotic. The pGlo plasmid can then be used to transform bacteria to give them this new trait.

plasmid? •A circular piece of autonomously replicating DNA •Originally evolved by bacteria •May express antibiotic resistance gene or be modified to express proteins of interest

Plasmid pGLO-GFP-1UAG from Dr. Chang Liu's lab contains the insert Green Fluorescent Protein with 1 UAG codon and is published in Integr Biol (Camb). 2016 Apr 18;8(4):542-5. doi: 10.1039/c5ib00232j. Epub 2015 Nov 27. This plasmid is available through Addgene.