Woodchuck Hepatitis Virus (WHV) Posttranscriptional Regulatory Element (WPRE) is a DNA sequence that, when transcribed, creates a tertiary structure enhancing expression. The …

Mar 17, 2025 · WPRE is defined by a highly structured RNA sequence that enhances gene expression through post-transcriptional mechanisms. Its function is attributed to a series of …

Oct 21, 2015 · What is WPRE? Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) is a DNA sequence that, when transcribed creates a tertiary structure …

The expression of genes delivered by retroviral vectors is often inefficient, a potential obstacle for their widespread use in human gene therapy. Here, we explored the possibility that the …

Jun 28, 2007 · WPRE is most effective when placed downstream of the transgene, proximal to the polyadenylation signal. We hypothesized that WPRE likely reduces viral mRNA readthrough …

Transgene expression levels can be modulated by engineering the expression cassette to include the CMV enhancer (that includes transcription factor binding sites) or mRNA stability/nuclear …

The woodchuck hepatitis virus has a post-transcriptional regulatory element (WPRE) and our investigators found that it can substantially stimulate the expression of transgenes delivered by …

May 10, 2006 · We designed a series of murine leukaemia virus (MLV)-based retroviral promoter conversion (ProCon) vectors that contain the woodchuck hepatitis post-transcriptional …

WPRE-mediated improvement is seen across several types of human and mouse primary and transformed cells and is translatable in vivo to the mouse liver. The woodchuck hepatitis virus …

Mar 5, 2009 · By providing additional evidence of efficacy of this WAS lentiviral vector with improved safety features, our results validate a mutated WPRE, which should be useful in …