Here, we report that VE-cadherin expression and junctional clustering are required for optimal transforming growth factor-β (TGF-β) signalling in endothelial cells (ECs). TGF-β antiproliferative and antimigratory responses are increased in the presence of VE-cadherin.

Other investigators have shown that exogenously administered transforming growth factor-beta (TGF-beta) inhibits lymphocyte adherence to vascular endothelial cells (VEC). We examined the role of TGF-beta 1 as an autocrine mediator of lymphocyte adhesion to adult human VECs.

We found that TGF-β expression is significantly increased after mesoderm induction, and inhibition of TGF-β enhances the development of CD34 + CD31 + VEC + hematopoietic and endothelial progenitors. Our study demonstrated that TGF-β signaling has the double-edged effect on hematopoietic and endothelial lineage specification.

Since 14-3-3ζ enhances TGF-β signaling and induces early transformation and EMT of human mammary epithelial cells (HMECs) (Lu et al., 2009), we used non-transformed MCF10A HMECs stably transfected with a control vector (10A.Vec) or 14-3-3ζ cDNA (10A. ζ). In 3D culture, the 10A.Vec cells produced normal acini, while the 10A.ζ cells ...

VEC clones underwent TGF-β 1-mediated EndMT, shown by significantly increased mRNA expression of the EndMT markers α-SMA (5.3±1.2), MMP-2 (13.5±0.6) and Slug (12±2.1) (p<0.05), (compared to unstimulated controls).

Apr 4, 2014 · To better understand valvular EndMT, we investigated TGFβ-induced signaling in mitral VEC, and carotid artery endothelial cells (CAEC) as a control. Expression of EndMT target genes α-smooth muscle actin (α-SMA), Snai1, Slug, and MMP-2 were used to monitor EndMT.

Jul 5, 2020 · Approach and results: VEC clones underwent TGF-β1-mediated EndMT, shown by significantly increased mRNA expression of the EndMT markers α-SMA (5.3 ± 1.2), MMP-2 (13.5 ± 0.6) and Slug (12 ± 2.1) (p < 0.05), (compared to unstimulated controls).

Oct 15, 2013 · These data provide evidence that a balance of positive and negative effects of TGF-β signaling at the appropriate timing is critical, and potential means to improve hematopoiesis and vasculogenesis from hPSCs.

Mar 26, 2020 · The use of OVs as anticancer agents is emerging and driven by the US FDA approval of talimogene laherparepvec (T-VEC), a modified herpes simplex virus type 1 (HSV-1) that increased survival and demonstrated favorable tolerability in patients with …

Mar 13, 2008 · Here, we report that VE‐cadherin expression and junctional clustering are required for optimal transforming growth factor‐β (TGF‐β) signalling in endothelial cells (ECs). TGF‐β antiproliferative and antimigratory responses are increased in the presence of VE‐cadherin.