Jan 4, 2011 · Inhibition of new protein synthesis by cycloheximide (CHX) can block the survival pathway and, thus, is widely used in studying cell death. Indeed, treatment of primary MEF cells with 30...

MODE-K cells are sensitive to TNF-α-induced apoptosis in the presence of CHX, which is associated with increased intracellular ROS production and caspase-3/7 activation. The effects were partially mitigated by CORM-A1, nitrite and resveratrol.

Mar 5, 2010 · As expected, TNF- α treatment increased ROS levels in cells pretreated with CHX (Figure 1a). Interestingly, the TNF- α -stimulated ROS increase was completely blocked by Romo1 knockdown.

Oct 22, 2018 · TNF-like weak inducer of apoptosis (TWEAK) and inhibition of protein synthesis with cycloheximide (CHX) sensitize for poly (I:C)-induced cell death. Notably, although CHX preferentially enhanced...

Ionophore A23187 (1 microM) protected cells against cell death at 12 and 24 but only partially at 6 hour of treatment. On the other hand, AD (10 ng/ml) acted synergistically with TNF-alpha and CHX at 6 and 12 hour.

It has been shown that necroptosis—caspase-independent programmed necrotic cell death—can be induced by treatment with tumor necrosis factor (TNF) in the L929 murine fibrosarcoma cell line, even in the absence of a caspase inhibitor.

Previously we have shown that both Rac1 and c-Jun NH(2)-terminal kinase (JNK1/2) are key proapoptotic molecules in tumor necrosis factor (TNF)-alpha/cycloheximide (CHX)-induced apoptosis in intestinal epithelial cells, whereas the role of reactive oxygen species (ROS) in apoptosis is unclear.

Jun 1, 2015 · TNF-α/CHX decreases cellular respiration and increases mitochondrial dysfunction. Mitochondria and NOX are major sources of ROS during TNF-α/CHX-induced cell death. Mitochondrial complexes I and II are the major sites of mitochondrial ROS production.

TNF co-treatment with cycloheximide (CHX) results in synthesis inhibition of a short living protein cFLIP, which is a known inhibitor of caspase-8. This leads to formation of the complex IIa and subsequent apoptosis [ 3 ].

As seen from Figure 1, cells treated with TNF and CHX undergo either apoptosis or necrosis when treated with either necrostatin-1 or zVAD, respectively. That's what everyone would expect.