A derivative of tetracycline, doxycycline (dox), is a preferred effector for tetracycline transregulation. Dox binds with high affinity to tTA and rtTA; thus, dox can be used in both …

We here review how a dox-controlled HIV-1 variant was designed and used to greatly improve the activity and dox-sensitivity of the rtTA transcriptional activator component of the Tet-On system.

Tetracycline-controlled transcriptional activation is a method of inducible gene expression where transcription is reversibly turned on or off in the presence of the antibiotic tetracycline or one of …

Apr 1, 2015 · In the absence of Tc or Dox, tTA binds to the TRE and activates transcription of the target gene. In the presence of Tc or Dox, tTA cannot bind to the TRE, and expression from …

Apr 1, 2002 · However, unlike tTA, tTS actively suppresses transcription of the target gene, preventing leaky expression of the transgene. In the presence of Dox, tTS dissociates from …

May 25, 2006 · In vivo use of the inducible tetracycline (Tc)-regulated gene expression system (Tet-On system) is limited by its low sensitivity for the effector doxycycline (dox). We used viral …

Here we describe the generation of two mouse strains that enable Cre-dependent, robust expression of rtTA3, providing tissue-restricted and consistent induction of TRE -controlled …

Jul 20, 2006 · Tet-on cell lines engineered to stably express doxycycline (Dox)-regulated reverse transcriptional transactivator (rtTA) have many applications in biomedical research and...

The doxycycline (dox)-inducible Tet-On system is widely used to control gene expression in mammalian cells. This system is based on the bacterial Tet operon, which has been modified …

Jun 21, 2016 · Two properties of rtTA have been targets for extensive optimization: increasing the sensitivity to doxycycline and increasing its dynamic range by reducing its basal activity in the …